3brg

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(New page: 200px {{Structure |PDB= 3brg |SIZE=350|CAPTION= <scene name='initialview01'>3brg</scene>, resolution 2.20&Aring; |SITE= <scene name='pdbsite=AC1:Edo+Binding+Site+...)
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[[Image:3brg.jpg|left|200px]]
[[Image:3brg.jpg|left|200px]]
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{{Structure
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|PDB= 3brg |SIZE=350|CAPTION= <scene name='initialview01'>3brg</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_3brg", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Edo+Binding+Site+For+Residue+C+1'>AC1</scene>, <scene name='pdbsite=AC2:Edo+Binding+Site+For+Residue+C+2'>AC2</scene>, <scene name='pdbsite=AC3:Edo+Binding+Site+For+Residue+C+3'>AC3</scene>, <scene name='pdbsite=AC4:Edo+Binding+Site+For+Residue+C+4'>AC4</scene>, <scene name='pdbsite=AC5:Edo+Binding+Site+For+Residue+C+5'>AC5</scene>, <scene name='pdbsite=AC6:Edo+Binding+Site+For+Residue+C+6'>AC6</scene>, <scene name='pdbsite=AC7:Edo+Binding+Site+For+Residue+C+7'>AC7</scene>, <scene name='pdbsite=AC8:Edo+Binding+Site+For+Residue+C+8'>AC8</scene>, <scene name='pdbsite=AC9:Edo+Binding+Site+For+Residue+C+9'>AC9</scene>, <scene name='pdbsite=BC1:Edo+Binding+Site+For+Residue+C+10'>BC1</scene>, <scene name='pdbsite=BC2:Edo+Binding+Site+For+Residue+C+11'>BC2</scene> and <scene name='pdbsite=BC3:Edo+Binding+Site+For+Residue+C+12'>BC3</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= Rbpj, Igkjrb1, Igkrsbp, Rbpsuh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam09271 LAG1-DNAbind], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01176 IPT_RBP-Jkappa], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam09270 Beta-trefoil]</span>
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{{STRUCTURE_3brg| PDB=3brg | SCENE= }}
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|RELATEDENTRY=[[3brd|3BRD]], [[3brf|3BRF]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3brg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3brg OCA], [http://www.ebi.ac.uk/pdbsum/3brg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3brg RCSB]</span>
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}}
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'''CSL (RBP-Jk) bound to DNA'''
'''CSL (RBP-Jk) bound to DNA'''
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==Overview==
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The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.
==About this Structure==
==About this Structure==
3BRG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRG OCA].
3BRG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRG OCA].
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==Reference==
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RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18381292 18381292]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Friedmann, D R.]]
[[Category: Friedmann, D R.]]
[[Category: Kovall, R A.]]
[[Category: Kovall, R A.]]
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[[Category: activator]]
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[[Category: Activator]]
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[[Category: alternative splicing]]
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[[Category: Alternative splicing]]
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[[Category: dna binding protein/dna complex]]
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[[Category: Dna binding protein/dna complex]]
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[[Category: dna-binding]]
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[[Category: Dna-binding]]
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[[Category: notch]]
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[[Category: Notch]]
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[[Category: notch signaling pathway]]
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[[Category: Notch signaling pathway]]
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[[Category: nucleus]]
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[[Category: Nucleus]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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[[Category: repressor]]
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[[Category: Repressor]]
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[[Category: signaling]]
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[[Category: Signaling]]
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[[Category: transcription]]
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[[Category: Transcription]]
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[[Category: transcription regulation]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:33:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:59:31 2008''
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Revision as of 06:33, 24 April 2008

Image:3brg.jpg

Template:STRUCTURE 3brg

CSL (RBP-Jk) bound to DNA


Overview

The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.

About this Structure

3BRG is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:18381292 Page seeded by OCA on Thu Apr 24 09:33:37 2008

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