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5tkz

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Current revision (15:41, 6 March 2024) (edit) (undo)
 
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==MEC-8 N-terminal RRM bound to tandem GCAC ligand==
==MEC-8 N-terminal RRM bound to tandem GCAC ligand==
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<StructureSection load='5tkz' size='340' side='right' caption='[[5tkz]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
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<StructureSection load='5tkz' size='340' side='right'caption='[[5tkz]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5tkz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TKZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5tkz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TKZ FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mec-8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.529&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tkz OCA], [http://pdbe.org/5tkz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tkz RCSB], [http://www.ebi.ac.uk/pdbsum/5tkz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tkz ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tkz OCA], [https://pdbe.org/5tkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tkz RCSB], [https://www.ebi.ac.uk/pdbsum/5tkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tkz ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/G5ECJ4_CAEEL G5ECJ4_CAEEL]
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Precise regulation of mRNA processing, translation, localization and stability relies on specific interactions with RNA-binding proteins whose biological function and target preference are dictated by their preferred RNA motifs. The RBPMS family of RNA-binding proteins is defined by a conserved RNA recognition motif (RRM) domain found in metazoan RBPMS/Hermes and RBPMS2, Drosophila couch potato, and MEC-8 from C. elegans. In order to determine the parameters of RNA sequence recognition by the RBPMS family, we have first used the N-terminal domain from MEC-8 in binding assays and have demonstrated a preference for two GCAC motifs optimally separated by greater than six nucleotides. We have also determined the crystal structure of the dimeric N-terminal RRM domain from MEC-8 in the unbound form, and in complex with an oligonucleotide harbouring two copies of the optimal GCAC motif. The atomic details reveal the molecular network that provides specificity to all four bases in the motif, including multiple hydrogen bonds to the initial guanine. Further studies with human RBPMS, as well as Drosophila couch potato, confirm a general preference for this double GCAC motif by other members of the protein family and the presence of this motif in known targets.
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Conserved binding of GCAC motifs by MEC-8, couch potato and the RBPMS protein family.,Soufari H, Mackereth CD RNA. 2016 Dec 21. pii: rna.059733.116. doi: 10.1261/rna.059733.116. PMID:28003515<ref>PMID:28003515</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5tkz" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Caeel]]
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[[Category: Caenorhabditis elegans]]
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[[Category: Mackereth, C D]]
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[[Category: Large Structures]]
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[[Category: Soufari, H]]
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[[Category: Synthetic construct]]
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[[Category: Alternative splicing]]
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[[Category: Mackereth CD]]
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[[Category: Dna]]
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[[Category: Soufari H]]
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[[Category: Elegan]]
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[[Category: Rrm]]
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[[Category: Splicing]]
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Current revision

MEC-8 N-terminal RRM bound to tandem GCAC ligand

PDB ID 5tkz

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