3wtd
From Proteopedia
(Difference between revisions)
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==Structure of PAXX== | ==Structure of PAXX== | ||
| - | <StructureSection load='3wtd' size='340' side='right' caption='[[3wtd]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='3wtd' size='340' side='right'caption='[[3wtd]], [[Resolution|resolution]] 2.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wtd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WTD FirstGlance]. <br> | <table><tr><td colspan='2'>[[3wtd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WTD FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wtd OCA], [http://pdbe.org/3wtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wtd RCSB], [http://www.ebi.ac.uk/pdbsum/3wtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wtd ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wtd OCA], [http://pdbe.org/3wtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wtd RCSB], [http://www.ebi.ac.uk/pdbsum/3wtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wtd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | XRCC4 and XLF are two structurally related proteins that function in DNA double-strand break (DSB) repair. Here, we identify human PAXX (PAralog of XRCC4 and XLF, also called C9orf142) as a new XRCC4 superfamily member and show that its crystal structure resembles that of XRCC4. PAXX interacts directly with the DSB-repair protein Ku and is recruited to DNA-damage sites in cells. Using RNA interference and CRISPR-Cas9 to generate PAXX(-/-) cells, we demonstrate that PAXX functions with XRCC4 and XLF to mediate DSB repair and cell survival in response to DSB-inducing agents. Finally, we reveal that PAXX promotes Ku-dependent DNA ligation in vitro and assembly of core nonhomologous end-joining (NHEJ) factors on damaged chromatin in cells. These findings identify PAXX as a new component of the NHEJ machinery. | ||
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| + | DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair.,Ochi T, Blackford AN, Coates J, Jhujh S, Mehmood S, Tamura N, Travers J, Wu Q, Draviam VM, Robinson CV, Blundell TL, Jackson SP Science. 2015 Jan 9;347(6218):185-188. doi: 10.1126/science.1261971. PMID:25574025<ref>PMID:25574025</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3wtd" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Blundell, T L]] | [[Category: Blundell, T L]] | ||
[[Category: Ochi, T]] | [[Category: Ochi, T]] | ||
Revision as of 08:17, 18 December 2019
Structure of PAXX
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