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5zql

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Revision as of 09:26, 30 October 2019

crystal structure of human katanin AAA ATPase domain

Structural highlights

5zql is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	KATNA1 (HUMAN)
Activity:	Microtubule-severing ATPase, with EC number 3.6.4.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KTNA1_HUMAN] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[HAMAP-Rule:MF_03023]^[1] ^[2] ^[3]

Publication Abstract from PubMed

Katanin was the first microtubule (MT)-severing enzyme discovered, but how katanin executes MT severing remains poorly understood. Here, we report X-ray crystal structures of the apo and ATPgammaS-bound states of the catalytic AAA domain of human katanin p60 at 3.0 and 2.9 A resolution, respectively. Comparison of the two structures reveals conformational changes induced by ATP binding and how such changes ensure hexamer stability. Moreover, we uncover structural details of pore loops (PLs) and show that Arg283, a residue unique to katanin among MT-severing enzymes, protrudes from PL1 and lines the entry of the catalytic pore. Functional studies suggest that PL1 and Arg283 play essential roles in the recognition and remodeling of the glutamylated, C-terminal tubulin tail and regulation of axon growth. In addition, domain-swapping experiments in katanin and spastin suggest that the non-homologous N-terminal region, which contains the MT-interacting and trafficking domain and a linker, confers specificity to the severing process.

Structural and Molecular Basis for Katanin-Mediated Severing of Glutamylated Microtubules.,Shin SC, Im SK, Jang EH, Jin KS, Hur EM, Kim EE Cell Rep. 2019 Jan 29;26(5):1357-1367.e5. doi: 10.1016/j.celrep.2019.01.020. PMID:30699360^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ McNally KP, Bazirgan OA, McNally FJ. Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin. J Cell Sci. 2000 May;113 ( Pt 9):1623-33. PMID:10751153
↑ Buster D, McNally K, McNally FJ. Katanin inhibition prevents the redistribution of gamma-tubulin at mitosis. J Cell Sci. 2002 Mar 1;115(Pt 5):1083-92. PMID:11870226
↑ Maddika S, Chen J. Protein kinase DYRK2 is a scaffold that facilitates assembly of an E3 ligase. Nat Cell Biol. 2009 Apr;11(4):409-19. doi: 10.1038/ncb1848. Epub 2009 Mar 15. PMID:19287380 doi:10.1038/ncb1848
↑ Shin SC, Im SK, Jang EH, Jin KS, Hur EM, Kim EE. Structural and Molecular Basis for Katanin-Mediated Severing of Glutamylated Microtubules. Cell Rep. 2019 Jan 29;26(5):1357-1367.e5. doi: 10.1016/j.celrep.2019.01.020. PMID:30699360 doi:http://dx.doi.org/10.1016/j.celrep.2019.01.020

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zql"

@@ Line 1: / Line 1: @@
 ==crystal structure of human katanin AAA ATPase domain==
-<StructureSection load='5zql' size='340' side='right' caption='[[5zql]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
+<StructureSection load='5zql' size='340' side='right'caption='[[5zql]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zql]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZQL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZQL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zql]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZQL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZQL FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KATNA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zql OCA], [http://pdbe.org/5zql PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zql RCSB], [http://www.ebi.ac.uk/pdbsum/5zql PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zql ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Microtubule-severing ATPase]]
 [[Category: Kim, E E]]

5zql

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Revision as of 09:26, 30 October 2019

crystal structure of human katanin AAA ATPase domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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