6a7f
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==The cryo-EM structure of filamentous bacteriophage IKe major coat protein p8 shell assembly.== | ==The cryo-EM structure of filamentous bacteriophage IKe major coat protein p8 shell assembly.== | ||
| - | <StructureSection load='6a7f' size='340' side='right' caption='[[6a7f]], [[Resolution|resolution]] 3.40Å' scene=''> | + | <StructureSection load='6a7f' size='340' side='right'caption='[[6a7f]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6a7f]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A7F FirstGlance]. <br> | <table><tr><td colspan='2'>[[6a7f]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A7F FirstGlance]. <br> | ||
| Line 8: | Line 8: | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/CAPSD_BPIKE CAPSD_BPIKE]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity). | [[http://www.uniprot.org/uniprot/CAPSD_BPIKE CAPSD_BPIKE]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The filamentous bacteriophage IKe infects Escherichia coli cells bearing IncN pili. We report the cryo-electron microscopy structure of the micrometer-long IKe viral particle at a resolution of 3.4 A. The major coat protein [protein 8 (p8)] consists of 47 residues that fold into a approximately 68-A-long helix. An atomic model of the coat protein was built. Five p8 helices in a horizontal layer form a pentamer, and symmetrically neighboring p8 layers form a right-handed helical cylinder having a rise per pentamer of 16.77 A and a twist of 38.52 degrees . The inner surface of the capsid cylinder is positively charged and has direct interactions with the encapsulated circular single-stranded DNA genome, which has an electron density consistent with an unusual left-handed helix structure. Similar to capsid structures of other filamentous viruses, strong capsid packing in the IKe particle is maintained by hydrophobic residues. Despite having a different length and large sequence differences from other filamentous phages, pi-pi interactions were found between Tyr9 of one p8 and Trp29 of a neighboring p8 in IKe that are similar to interactions observed in phage M13, suggesting that, despite sequence divergence, overall structural features are maintained. | ||
| + | |||
| + | Cryo-electron microscopy structure of the filamentous bacteriophage IKe.,Xu J, Dayan N, Goldbourt A, Xiang Y Proc Natl Acad Sci U S A. 2019 Feb 28. pii: 1811929116. doi:, 10.1073/pnas.1811929116. PMID:30819888<ref>PMID:30819888</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6a7f" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Filamentous phage]] | [[Category: Filamentous phage]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Dayan, N]] | [[Category: Dayan, N]] | ||
[[Category: Goldbourt, A]] | [[Category: Goldbourt, A]] | ||
Revision as of 12:34, 13 March 2019
The cryo-EM structure of filamentous bacteriophage IKe major coat protein p8 shell assembly.
| |||||||||||
