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6h5t
From Proteopedia
(Difference between revisions)
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==Intersectin SH3A short isoform== | ==Intersectin SH3A short isoform== | ||
| - | <StructureSection load='6h5t' size='340' side='right' caption='[[6h5t]], [[Resolution|resolution]] 1.69Å' scene=''> | + | <StructureSection load='6h5t' size='340' side='right'caption='[[6h5t]], [[Resolution|resolution]] 1.69Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6h5t]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6h5t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5T FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7PG:2,5,8,11,14,17,20,23-OCTAOXAPENTACOSAN-25-OL'>7PG</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7PG:2,5,8,11,14,17,20,23-OCTAOXAPENTACOSAN-25-OL'>7PG</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ITSN1, ITSN, SH3D1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5t OCA], [http://pdbe.org/6h5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5t RCSB], [http://www.ebi.ac.uk/pdbsum/6h5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5t ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5t OCA], [http://pdbe.org/6h5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5t RCSB], [http://www.ebi.ac.uk/pdbsum/6h5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/ITSN1_HUMAN ITSN1_HUMAN]] Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.<ref>PMID:11744688</ref> <ref>PMID:22648170</ref> | [[http://www.uniprot.org/uniprot/ITSN1_HUMAN ITSN1_HUMAN]] Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.<ref>PMID:11744688</ref> <ref>PMID:22648170</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The scaffolding protein intersectin 1 plays important roles in clathrin-mediated endocytosis and in the replenishment of release-ready synaptic vesicles (SV). Two splice variants of intersectin's SH3A domain are expressed in the brain, and association of the neuron-specific variant with synapsin I has been shown to enable sustained neurotransmission and to be regulated by an adjacent C-terminal motif. Here, we demonstrate that the ubiquitously expressed short SH3A variant of intersectin 1 interacts with an N-terminal intramolecular sequence that operates synergistically with the C-terminal motif. NMR spectroscopic investigations show that the five-amino acid insertion into the beta strand 2 of the neuronal SH3A variant introduces conformational plasticity incompatible with binding of the N-terminal sequence. The difference in the autoregulatory mechanism of the domain's variants differentially affects its synaptic binding partners, thereby establishing alternative splicing in conjunction with autoinhibitory motif variation as a mechanism to regulate protein interaction networks. | ||
| + | |||
| + | Exon Inclusion Modulates Conformational Plasticity and Autoinhibition of the Intersectin 1 SH3A Domain.,Gerth F, Japel M, Sticht J, Kuropka B, Schmitt XJ, Driller JH, Loll B, Wahl MC, Pagel K, Haucke V, Freund C Structure. 2019 Apr 9. pii: S0969-2126(19)30116-9. doi:, 10.1016/j.str.2019.03.020. PMID:31031201<ref>PMID:31031201</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6h5t" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Driller, J H]] | [[Category: Driller, J H]] | ||
[[Category: Freund, C]] | [[Category: Freund, C]] | ||
Current revision
Intersectin SH3A short isoform
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Categories: Human | Large Structures | Driller, J H | Freund, C | Gerth, F | Loll, B | Wahl, M C | Endocytosis | Intersectin 1 | Sh3 domain | Splice isoform
