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5al7

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Revision as of 06:12, 31 May 2023

N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain.

Structural highlights

5al7 is a 2 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAS6_DROME Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication.^[1] ^[2]

Publication Abstract from PubMed

Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the nine-fold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coiled-Coil Domain (CCCD). Here we show that the Drosophila Ana2 CCCD forms a tetramer, and we solve its structure to 0.8 A, revealing that it adopts an unusual parallel-coil topology. We also solve the structure of the Drosophila Sas-6 N-terminal domain to 2.9 A revealing that it forms higher-order oligomers through canonical interactions. Point mutations that perturb Sas-6 or Ana2 homo-oligomerisation in vitro strongly perturb centriole assembly in vivo. Thus, efficient centriole duplication in flies requires the homo-oligomerisation of both Sas-6 and Ana2, and the Ana2 CCCD tetramer structure provides important information on how these proteins might cooperate to form a cartwheel structure.

The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.,Cottee MA, Muschalik N, Johnson S, Leveson J, Raff JW, Lea SM Elife. 2015 May 23;4. doi: 10.7554/eLife.07236. PMID:26002084^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Goshima G, Wollman R, Goodwin SS, Zhang N, Scholey JM, Vale RD, Stuurman N. Genes required for mitotic spindle assembly in Drosophila S2 cells. Science. 2007 Apr 20;316(5823):417-21. Epub 2007 Apr 5. PMID:17412918 doi:http://dx.doi.org/1141314
↑ Rodrigues-Martins A, Riparbelli M, Callaini G, Glover DM, Bettencourt-Dias M. Revisiting the role of the mother centriole in centriole biogenesis. Science. 2007 May 18;316(5827):1046-50. Epub 2007 Apr 26. PMID:17463247 doi:http://dx.doi.org/10.1126/science.1142950
↑ Cottee MA, Muschalik N, Johnson S, Leveson J, Raff JW, Lea SM. The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. Elife. 2015 May 23;4. doi: 10.7554/eLife.07236. PMID:26002084 doi:http://dx.doi.org/10.7554/eLife.07236

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5al7"

Categories: Drosophila melanogaster | Large Structures | Cottee MA | Johnson S | Lea SM

@@ Line 1: / Line 1: @@
 ==N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain.==
-<StructureSection load='5al7' size='340' side='right' caption='[[5al7]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
+<StructureSection load='5al7' size='340' side='right'caption='[[5al7]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5al7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AL7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5al7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AL7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5al6|5al6]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5al7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5al7 OCA], [https://pdbe.org/5al7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5al7 RCSB], [https://www.ebi.ac.uk/pdbsum/5al7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5al7 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5al7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5al7 OCA], [http://pdbe.org/5al7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5al7 RCSB], [http://www.ebi.ac.uk/pdbsum/5al7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5al7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SAS6_DROME SAS6_DROME]] Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication.<ref>PMID:17412918</ref> <ref>PMID:17463247</ref>
+[https://www.uniprot.org/uniprot/SAS6_DROME SAS6_DROME] Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication.<ref>PMID:17412918</ref> <ref>PMID:17463247</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
-[[Category: Cottee, M A]]
+[[Category: Large Structures]]
-[[Category: Johnson, S]]
+[[Category: Cottee MA]]
-[[Category: Lea, S M]]
+[[Category: Johnson S]]
-[[Category: Cartwheel]]
+[[Category: Lea SM]]
-[[Category: Centriole]]
-[[Category: Structural protein]]

5al7

From Proteopedia

Revision as of 06:12, 31 May 2023

N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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