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| | ==PrgI-SipD from Salmonella typhimurium== | | ==PrgI-SipD from Salmonella typhimurium== |
| - | <StructureSection load='3zqb' size='340' side='right' caption='[[3zqb]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='3zqb' size='340' side='right'caption='[[3zqb]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zqb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zqb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQB FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ym9|2ym9]], [[2ym0|2ym0]], [[2x9c|2x9c]], [[3zqe|3zqe]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ym9|2ym9]], [[2ym0|2ym0]], [[2x9c|2x9c]], [[3zqe|3zqe]]</div></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqb OCA], [http://pdbe.org/3zqb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zqb RCSB], [http://www.ebi.ac.uk/pdbsum/3zqb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqb ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqb OCA], [https://pdbe.org/3zqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqb RCSB], [https://www.ebi.ac.uk/pdbsum/3zqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY]] Required for invasion of epithelial cells. | + | [[https://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY]] Required for invasion of epithelial cells. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Salty]] | | [[Category: Salty]] |
| | [[Category: Kolbe, M]] | | [[Category: Kolbe, M]] |
| Structural highlights
Function
[PRGI_SALTY] Required for invasion of epithelial cells.
Publication Abstract from PubMed
Many infectious gram-negative bacteria, including Salmonella typhimurium, require a Type Three Secretion System (T3SS) to translocate virulence factors into host cells. The T3SS consists of a membrane protein complex and an extracellular needle together that form a continuous channel. Regulated secretion of virulence factors requires the presence of SipD at the T3SS needle tip in S. typhimurium. Here we report three-dimensional structures of individual SipD, SipD in fusion with the needle subunit PrgI, and of SipD:PrgI in complex with the bile salt, deoxycholate. Assembly of the complex involves major conformational changes in both SipD and PrgI. This rearrangement is mediated via a pi bulge in the central SipD helix and is stabilized by conserved amino acids that may allow for specificity in the assembly and composition of the tip proteins. Five copies each of the needle subunit PrgI and SipD form the T3SS needle tip complex. Using surface plasmon resonance spectroscopy and crystal structure analysis we found that the T3SS needle tip complex binds deoxycholate with micromolar affinity via a cleft formed at the SipD:PrgI interface. In the structure-based three-dimensional model of the T3SS needle tip, the bound deoxycholate faces the host membrane. Recently, binding of SipD with bile salts present in the gut was shown to impede bacterial infection. Binding of bile salts to the SipD:PrgI interface in this particular arrangement may thus inhibit the T3SS function. The structures presented in this study provide insight into the open state of the T3SS needle tip. Our findings present the atomic details of the T3SS arrangement occurring at the pathogen-host interface.
Crystal structure of PrgI-SipD: insight into a secretion competent state of the type three secretion system needle tip and its interaction with host ligands.,Lunelli M, Hurwitz R, Lambers J, Kolbe M PLoS Pathog. 2011 Aug;7(8):e1002163. Epub 2011 Aug 4. PMID:21829362[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lunelli M, Hurwitz R, Lambers J, Kolbe M. Crystal structure of PrgI-SipD: insight into a secretion competent state of the type three secretion system needle tip and its interaction with host ligands. PLoS Pathog. 2011 Aug;7(8):e1002163. Epub 2011 Aug 4. PMID:21829362 doi:10.1371/journal.ppat.1002163
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