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6mzb
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of phosphodiesterase 6== | ==Cryo-EM structure of phosphodiesterase 6== | ||
| - | <StructureSection load='6mzb' size='340' side='right' | + | <StructureSection load='6mzb' size='340' side='right'caption='[[6mzb]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6mzb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MZB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mzb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MZB FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=35G:GUANOSINE-3,5-MONOPHOSPHATE'>35G</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=35G:GUANOSINE-3,5-MONOPHOSPHATE'>35G</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PDE6B, PDEB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN]), PDE6A, PDEA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN]), PDE6G, PDEG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3',5'-cyclic-GMP_phosphodiesterase 3',5'-cyclic-GMP phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.35 3.1.4.35] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3',5'-cyclic-GMP_phosphodiesterase 3',5'-cyclic-GMP phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.35 3.1.4.35] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzb OCA], [http://pdbe.org/6mzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mzb RCSB], [http://www.ebi.ac.uk/pdbsum/6mzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzb ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzb OCA], [http://pdbe.org/6mzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mzb RCSB], [http://www.ebi.ac.uk/pdbsum/6mzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzb ProSAT]</span></td></tr> | ||
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/PDE6B_BOVIN PDE6B_BOVIN]] This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme. [[http://www.uniprot.org/uniprot/CNRG_BOVIN CNRG_BOVIN]] Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones. [[http://www.uniprot.org/uniprot/PDE6A_BOVIN PDE6A_BOVIN]] This protein participates in processes of transmission and amplification of the visual signal. | [[http://www.uniprot.org/uniprot/PDE6B_BOVIN PDE6B_BOVIN]] This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme. [[http://www.uniprot.org/uniprot/CNRG_BOVIN CNRG_BOVIN]] Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones. [[http://www.uniprot.org/uniprot/PDE6A_BOVIN PDE6A_BOVIN]] This protein participates in processes of transmission and amplification of the visual signal. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cyclic nucleotide phosphodiesterases (PDEs) work in conjunction with adenylate/guanylate cyclases to regulate the key second messengers of G protein-coupled receptor signaling. Previous attempts to determine the full-length structure of PDE family members at high-resolution have been hindered by structural flexibility, especially in their linker regions and N- and C-terminal ends. Therefore, most structure-activity relationship studies have so far focused on truncated and conserved catalytic domains rather than the regulatory domains that allosterically govern the activity of most PDEs. Here, we used single-particle cryo-electron microscopy to determine the structure of the full-length PDE6alphabeta2gamma complex. The final density map resolved at 3.4 A reveals several previously unseen structural features, including a coiled N-terminal domain and the interface of PDE6gamma subunits with the PDE6alphabeta heterodimer. Comparison of the PDE6alphabeta2gamma complex with the closed state of PDE2A sheds light on the conformational changes associated with the allosteric activation of type I PDEs. | ||
| + | |||
| + | Cryo-EM structure of phosphodiesterase 6 reveals insights into the allosteric regulation of type I phosphodiesterases.,Gulati S, Palczewski K, Engel A, Stahlberg H, Kovacik L Sci Adv. 2019 Feb 27;5(2):eaav4322. doi: 10.1126/sciadv.aav4322. eCollection 2019, Feb. PMID:30820458<ref>PMID:30820458</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6mzb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: 3',5'-cyclic-GMP phosphodiesterase]] | [[Category: 3',5'-cyclic-GMP phosphodiesterase]] | ||
| + | [[Category: Bovin]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Gulati, S]] | [[Category: Gulati, S]] | ||
[[Category: Palczewski, K]] | [[Category: Palczewski, K]] | ||
Revision as of 12:47, 13 March 2019
Cryo-EM structure of phosphodiesterase 6
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