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6hns

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Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+

Structural highlights

6hns is a 2 chain structure with sequence from Nitrincola lacisaponensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.84Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A063Y6V3_9GAMM

Publication Abstract from PubMed

The flavin-containing monooxygenases (FMOs) play an important role in drug metabolism but they also have a high potential in industrial biotransformations. Among the hitherto characterized FMOs, there was no thermostable representative, while such biocatalyst would be valuable for FMO-based applications. Through a targeted genome mining approach, we have identified a gene encoding for a putative FMO from Nitrincola lacisaponensis, an alkaliphilic extremophile bacterium. Herein, we report the biochemical and structural characterization of this newly discovered bacterial FMO (NiFMO). NiFMO can be expressed as active and soluble enzyme at high level in Escherichia coli (90-100 mg/L of culture). NiFMO is relatively thermostable (melting temperature (Tm) of 51 degrees C), displays high organic solvent tolerance, and accepts a broad range of substrates. The crystal structure of NiFMO was solved at 1.8 A resolution, which allows future structure-based enzyme engineering. Altogether, NiFMO represents an interesting newly discovered enzyme with the appropriate features to develop into an industrially applied biocatalyst.

Characterization of a thermostable flavin-containing monooxygenase from Nitrincola lacisaponensis (NiFMO).,Loncar N, Fiorentini F, Bailleul G, Savino S, Romero E, Mattevi A, Fraaije MW Appl Microbiol Biotechnol. 2019 Jan 4. pii: 10.1007/s00253-018-09579-w. doi:, 10.1007/s00253-018-09579-w. PMID:30607493^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Loncar N, Fiorentini F, Bailleul G, Savino S, Romero E, Mattevi A, Fraaije MW. Characterization of a thermostable flavin-containing monooxygenase from Nitrincola lacisaponensis (NiFMO). Appl Microbiol Biotechnol. 2019 Jan 4. pii: 10.1007/s00253-018-09579-w. doi:, 10.1007/s00253-018-09579-w. PMID:30607493 doi:http://dx.doi.org/10.1007/s00253-018-09579-w

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6hns"

Categories: Large Structures | Nitrincola lacisaponensis | Fiorentini F | Mattevi A

@@ Line 1: / Line 1: @@
 ==Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+==
-<StructureSection load='6hns' size='340' side='right'  caption='[[6hns]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
+<StructureSection load='6hns' size='340' side='right'caption='[[6hns]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-920 Atcc baa-920]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HNS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6hns]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrincola_lacisaponensis Nitrincola lacisaponensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HNS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ADINL_0099 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=267850 ATCC BAA-920])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [http://pdbe.org/6hns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [http://www.ebi.ac.uk/pdbsum/6hns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hns OCA], [https://pdbe.org/6hns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hns RCSB], [https://www.ebi.ac.uk/pdbsum/6hns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hns ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A063Y6V3_9GAMM A0A063Y6V3_9GAMM]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 6hns" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Atcc baa-920]]
+[[Category: Large Structures]]
-[[Category: Fiorentini, F]]
+[[Category: Nitrincola lacisaponensis]]
-[[Category: Mattevi, A]]
+[[Category: Fiorentini F]]
-[[Category: Drug metabolism]]
+[[Category: Mattevi A]]
-[[Category: Flavoprotein]]
-[[Category: Indigo]]
-[[Category: Monooxygenase]]
-[[Category: Thermostability]]

6hns

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Current revision

Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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