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| | <StructureSection load='4aqs' size='340' side='right'caption='[[4aqs]], [[Resolution|resolution]] 3.11Å' scene=''> | | <StructureSection load='4aqs' size='340' side='right'caption='[[4aqs]], [[Resolution|resolution]] 3.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4aqs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4aqs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AQS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4aqt|4aqt]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4aqt|4aqt]]</div></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqs OCA], [http://pdbe.org/4aqs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4aqs RCSB], [http://www.ebi.ac.uk/pdbsum/4aqs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4aqs ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqs OCA], [https://pdbe.org/4aqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aqs RCSB], [https://www.ebi.ac.uk/pdbsum/4aqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aqs ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LAMB1_MOUSE LAMB1_MOUSE]] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of the cerebral cortex (By similarity). It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons (By similarity). Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface (By similarity). | + | [[https://www.uniprot.org/uniprot/LAMB1_MOUSE LAMB1_MOUSE]] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of the cerebral cortex (By similarity). It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons (By similarity). Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4aqs" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4aqs" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Laminin|Laminin]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| | [[Category: Carafoli, F]] | | [[Category: Carafoli, F]] |
| | [[Category: Hohenester, E]] | | [[Category: Hohenester, E]] |
| | [[Category: Hussain, S]] | | [[Category: Hussain, S]] |
| | [[Category: Cell adhesion]] | | [[Category: Cell adhesion]] |
| Structural highlights
Function
[LAMB1_MOUSE] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of the cerebral cortex (By similarity). It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons (By similarity). Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface (By similarity).
Publication Abstract from PubMed
The heterotrimeric laminins are a defining component of basement membranes and essential for tissue formation and function in all animals. The three short arms of the cross-shaped laminin molecule are composed of one chain each and their tips mediate the formation of a polymeric network. The structural basis for laminin polymerisation is unknown. We have determined crystal structures of the short-arm tips of the mouse laminin beta1 and gamma1 chains, which are grossly similar to the previously determined structure of the corresponding alpha5 chain region. The short-arm tips consist of a laminin N-terminal (LN) domain that is attached like the head of a flower to a rod-like stem formed by tandem laminin-type epidermal growth factor-like (LE) domains. The LN domain is a beta-sandwich with elaborate loop regions that differ between chains. The gamma1 LN domain uniquely contains a calcium binding site. The LE domains have little regular structure and are stabilised by cysteines that are disulphide-linked 1-3, 2-4, 5-6 and 7-8 in all chains. The LN surface is not conserved across the alpha, beta and gamma chains, but within each chain subfamily there is a striking concentration of conserved residues on one face of the beta-sandwich, while the opposite face invariably is shielded by glycans. We propose that the extensive conserved patches on the beta and gamma LN domains mediate the binding of these two chains to each other, and that the alpha chain LN domain subsequently binds to the composite beta-gamma surface. Mutations in the laminin beta2 LN domain causing Pierson syndrome are likely to impair the folding of the beta2 chain or its ability to form network interactions.
Crystal Structures of the Network-Forming Short-Arm Tips of the Laminin beta1 and gamma1 Chains.,Carafoli F, Hussain SA, Hohenester E PLoS One. 2012;7(7):e42473. Epub 2012 Jul 31. PMID:22860131[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Carafoli F, Hussain SA, Hohenester E. Crystal Structures of the Network-Forming Short-Arm Tips of the Laminin beta1 and gamma1 Chains. PLoS One. 2012;7(7):e42473. Epub 2012 Jul 31. PMID:22860131 doi:10.1371/journal.pone.0042473
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