6a4d

<table><tr><td colspan='2'>[[6a4d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A4D FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9RC:5-O-[(S)-hydroxy(4-nitrophenoxy)phosphoryl]thymidine'>9RC</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a4d OCA], [http://pdbe.org/6a4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a4d RCSB], [http://www.ebi.ac.uk/pdbsum/6a4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a4d ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/ORN_COLP3 ORN_COLP3]] 3'-to-5' exoribonuclease specific for small oligoribonucleotides.

Cells regulate their intracellular mRNA levels by using specific ribonucleases. Oligoribonuclease (ORN) is a 3'-5' exoribonuclease for small RNA molecules, important in RNA degradation and re-utilisation. However, there is no structural information on the ligand-binding form of ORNs. In this study, the crystal structures of oligoribonuclease from Colwellia psychrerythraea strain 34H (CpsORN) were determined in four different forms: unliganded-structure, thymidine 5'-monophosphate p-nitrophenyl ester (pNP-TMP)-bound, two separated uridine-bound, and two linked uridine (U-U)-bound forms. The crystal structures show that CpsORN is a tight dimer, with two separated active sites and one divalent metal cation ion in each active site. These structures represent several snapshots of the enzymatic reaction process, which allowed us to suggest a possible one-metal-dependent reaction mechanism for CpsORN. Moreover, the biochemical data support our suggested mechanism and identified the key residues responsible for enzymatic catalysis of CpsORN.

 

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== References ==

[[Category: Lee, C W]]

[[Category: Lee, J H]]

[[Category: Park, S]]

[[Category: Oligoribonuclease]]