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Publication Abstract from PubMed

This paper focuses on the nucleoprotein (NP) of the newly identified member of the Orthomyxoviridae family, Influenza D virus. To date several X-ray structures of NP of Influenza A (A/NP) and B (B/NP) viruses and of infectious salmon anemia (ISA/NP) virus have been solved. Here we purified, characterized and solved the X-ray structure of the tetrameric D/NP at 2.4 A resolution. The crystal structure of its core is similar to NP of other Influenza viruses. However, unlike A/NP and B/NP which possess a flexible amino-terminal tail containing nuclear localization signals (NLS) for their nuclear import, D/NP possesses a carboxy-terminal tail (D/NPTAIL). We show that D/NPTAIL harbors a bipartite NLS and designed C-terminal truncated mutants to demonstrate the role of D/NPTAIL for nuclear transport.

The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport.,Donchet A, Oliva J, Labaronne A, Tengo L, Miloudi M, C A Gerard F, Mas C, Schoehn G, W H Ruigrok R, Ducatez M, Crepin T Sci Rep. 2019 Jan 24;9(1):600. doi: 10.1038/s41598-018-37306-y. PMID:30679709^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.