This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4bwp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 3: Line 3:
<StructureSection load='4bwp' size='340' side='right'caption='[[4bwp]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
<StructureSection load='4bwp' size='340' side='right'caption='[[4bwp]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4bwp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BWP FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4bwp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BWP FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AN2:AMP+PHOSPHORAMIDATE'>AN2</scene></td></tr>
+
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AN2:AMP+PHOSPHORAMIDATE'>AN2</scene></td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bwk|4bwk]], [[4bwx|4bwx]]</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwp OCA], [https://pdbe.org/4bwp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bwp RCSB], [https://www.ebi.ac.uk/pdbsum/4bwp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwp ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwp OCA], [http://pdbe.org/4bwp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bwp RCSB], [http://www.ebi.ac.uk/pdbsum/4bwp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwp ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[[https://www.uniprot.org/uniprot/PAN3_DROME PAN3_DROME]] Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[HAMAP-Rule:MF_03181]<ref>PMID:23172285</ref>
-
The PAN2-PAN3 deadenylase complex functions in general and miRNA-mediated mRNA degradation and is specifically recruited to miRNA targets by GW182/TNRC6 proteins. We describe the PAN3 adaptor protein crystal structure that, unexpectedly, forms intertwined and asymmetric homodimers. Dimerization is mediated by a coiled coil that links an N-terminal pseudokinase to a C-terminal knob domain. The PAN3 pseudokinase binds ATP, and this function is required for mRNA degradation in vivo. We further identified conserved surfaces required for mRNA degradation, including the binding surface for the PAN2 deadenylase on the knob domain. The most remarkable structural feature is the presence of a tryptophan-binding pocket at the dimer interface, which mediates binding to TNRC6C in human cells. Together, our data reveal the structural basis for the interaction of PAN3 with PAN2 and the recruitment of the PAN2-PAN3 complex to miRNA targets by TNRC6 proteins.
+
-
 
+
-
Structure of the PAN3 Pseudokinase Reveals the Basis for Interactions with the PAN2 Deadenylase and the GW182 Proteins.,Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E Mol Cell. 2013 Aug 8;51(3):360-73. doi: 10.1016/j.molcel.2013.07.011. PMID:23932717<ref>PMID:23932717</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4bwp" style="background-color:#fffaf0;"></div>
+
==See Also==
==See Also==
-
*[[Ribonuclease|Ribonuclease]]
+
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Drome]]
+
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Boland, A]]
+
[[Category: Boland A]]
-
[[Category: Christie, M]]
+
[[Category: Christie M]]
-
[[Category: Huntzinger, E]]
+
[[Category: Huntzinger E]]
-
[[Category: Izaurralde, E]]
+
[[Category: Izaurralde E]]
-
[[Category: Weichenrieder, O]]
+
[[Category: Weichenrieder O]]
-
[[Category: Deadenylase]]
+
-
[[Category: Gene regulation]]
+
-
[[Category: Mirna]]
+
-
[[Category: Mrna decay]]
+
-
[[Category: Pan2]]
+

Revision as of 17:13, 7 September 2022

Structure of Drosophila Melanogaster PAN3 pseudokinase

PDB ID 4bwp

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bwp"
Personal tools