4c2u

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<StructureSection load='4c2u' size='340' side='right'caption='[[4c2u]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
<StructureSection load='4c2u' size='340' side='right'caption='[[4c2u]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C2U FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C2U FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c2t|4c2t]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [https://pdbe.org/4c2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [https://www.ebi.ac.uk/pdbsum/4c2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c2u ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [http://pdbe.org/4c2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [http://www.ebi.ac.uk/pdbsum/4c2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c2u ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/Q9RTI9_DEIRA Q9RTI9_DEIRA]]
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DNA helicases are responsible for unwinding the duplex DNA, a key step in many biological processes. UvrD is a DNA helicase involved in several DNA repair pathways. We report here crystal structures of Deinococcus radiodurans UvrD (drUvrD) in complex with DNA in different nucleotide-free and bound states. These structures provide us with three distinct snapshots of drUvrD in action and for the first time trap a DNA helicase undergoing a large-scale spiral movement around duplexed DNA. Our structural data also improve our understanding of the molecular mechanisms that regulate DNA unwinding by Superfamily 1A (SF1A) helicases. Our biochemical data reveal that drUvrD is a DNA-stimulated ATPase, can translocate along ssDNA in the 3'-5' direction and shows ATP-dependent 3'-5', and surprisingly also, 5'-3' helicase activity. Interestingly, we find that these translocase and helicase activities of drUvrD are modulated by the ssDNA binding protein. Analysis of drUvrD mutants indicate that the conserved beta-hairpin structure of drUvrD that functions as a separation pin is critical for both drUvrD's 3'-5' and 5'-3' helicase activities, whereas the GIG motif of drUvrD involved in binding to the DNA duplex is essential for the 5'-3' helicase activity only. These special features of drUvrD may reflect its involvement in a wide range of DNA repair processes in vivo.
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Structural and Mechanistic Insight into DNA Unwinding by Deinococcus radiodurans UvrD.,Stelter M, Acajjaoui S, McSweeney S, Timmins J PLoS One. 2013 Oct 15;8(10):e77364. doi: 10.1371/journal.pone.0077364. PMID:24143224<ref>PMID:24143224</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4c2u" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Helicase|Helicase]]
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Micrococcus radiodurans raj et al. 1960]]
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[[Category: Deinococcus radiodurans]]
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[[Category: DNA helicase]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Acajjaoui, S]]
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[[Category: Synthetic construct]]
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[[Category: McSweeney, S]]
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[[Category: Acajjaoui S]]
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[[Category: Stelter, M]]
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[[Category: McSweeney S]]
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[[Category: Timmins, J]]
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[[Category: Stelter M]]
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[[Category: Dna helicase]]
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[[Category: Timmins J]]
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[[Category: Dna repair]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Nucleotide excision repair]]
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Revision as of 17:22, 7 September 2022

Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1

PDB ID 4c2u

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