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6gv9
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the type IV pilus from enterohemorrhagic Escherichia coli (EHEC)== | |
| + | <StructureSection load='6gv9' size='340' side='right'caption='[[6gv9]], [[Resolution|resolution]] 8.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gv9]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GV9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6gms|6gms]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ECs0112 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gv9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gv9 OCA], [http://pdbe.org/6gv9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gv9 RCSB], [http://www.ebi.ac.uk/pdbsum/6gv9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gv9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial type 4a pili are dynamic surface filaments that promote bacterial adherence, motility, and macromolecular transport. Their genes are highly conserved among enterobacteria and their expression in enterohemorrhagic Escherichia coli (EHEC) promotes adhesion to intestinal epithelia and pro-inflammatory signaling. To define the molecular basis of EHEC pilus assembly, we determined the structure of the periplasmic domain of its major subunit PpdD (PpdDp), a prototype of an enterobacterial pilin subfamily containing two disulfide bonds. The structure of PpdDp, determined by NMR, was then docked into the density envelope of purified EHEC pili obtained by cryoelectron microscopy (cryo-EM). Cryo-EM reconstruction of EHEC pili at approximately 8 A resolution revealed extremely high pilus flexibility correlating with a large extended region of the pilin stem. Systematic mutagenesis combined with functional and interaction analyses identified charged residues essential for pilus assembly. Structural information on exposed regions and interfaces between EHEC pilins is relevant for vaccine and drug discovery. | ||
| - | + | Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.,Bardiaux B, de Amorim GC, Luna Rico A, Zheng W, Guilvout I, Jollivet C, Nilges M, Egelman EH, Izadi-Pruneyre N, Francetic O Structure. 2019 Apr 9. pii: S0969-2126(19)30117-0. doi:, 10.1016/j.str.2019.03.021. PMID:31056419<ref>PMID:31056419</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6gv9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Eco57]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Amorim, G C]] | ||
| + | [[Category: Bardiaux, B]] | ||
[[Category: Egelman, E]] | [[Category: Egelman, E]] | ||
| - | [[Category: Amorim, G.C]] | ||
| - | [[Category: Jollivet, C]] | ||
| - | [[Category: Izadi-Pruneyre, N]] | ||
[[Category: Francetic, O]] | [[Category: Francetic, O]] | ||
| - | [[Category: Nilges, M]] | ||
[[Category: Guilvout, I]] | [[Category: Guilvout, I]] | ||
| - | [[Category: | + | [[Category: Izadi-Pruneyre, N]] |
| + | [[Category: Jollivet, C]] | ||
[[Category: Luna-Rico, A]] | [[Category: Luna-Rico, A]] | ||
| - | [[Category: | + | [[Category: Nilges, M]] |
| + | [[Category: Zheng, W]] | ||
| + | [[Category: Protein fibril]] | ||
Revision as of 07:25, 21 May 2019
Structure of the type IV pilus from enterohemorrhagic Escherichia coli (EHEC)
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