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2jx2

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{{STRUCTURE_2jx2| PDB=2jx2 | SCENE= }}
{{STRUCTURE_2jx2| PDB=2jx2 | SCENE= }}
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'''Solution conformation of RNA-bound NELF-E RRM'''
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===Solution conformation of RNA-bound NELF-E RRM===
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==Overview==
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The E subunit of the human heterotetrameric negative transcription elongation factor (NELF-E) contains a canonical betaalphabetabetaalphabeta RNA recognition motif (RRM) that binds to a wide variety of RNA sequences. These induce very similar conformational changes in the RRM as determined by nuclear magnetic resonance spectroscopy. Although the RNA binding interface of a canonical RRM is mainly located at its beta-sheet surface, for NELF-E RRM large chemical shift perturbations are observed for residues in the flexible C-terminal region and the loop between beta 3 and alpha 2, and both regions are distant from the interface. We determined the solution structure of single-stranded transactivator responsive element (TAR) RNA-bound NELF-E RRM. This structure clearly shows that RNA binding to NELF-E RRM induces formation of a helix in the C-terminus. The RNA-bound form of NELF-E RRM is very similar to the RNA-bound form of U1A RRM, although the C-terminus of the NELF-E RRM is unstructured in the free protein, whereas it is helical in the U1A protein. Thus, RNA binding to NELF-E RRM induces a conformational change toward the U1A structure, resulting in highly similar RNA binding conformations for both proteins.
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{{ABSTRACT_PUBMED_18303858}}
==About this Structure==
==About this Structure==
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2JX2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JX2 OCA].
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2JX2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JX2 OCA].
==Reference==
==Reference==
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[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
[[Category: Ubl conjugation]]
[[Category: Ubl conjugation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:02:55 2008''

Revision as of 12:02, 28 July 2008

Image:2jx2.png

Template:STRUCTURE 2jx2

Solution conformation of RNA-bound NELF-E RRM

Template:ABSTRACT PUBMED 18303858

About this Structure

2JX2 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

NELF-E RRM Undergoes Major Structural Changes in Flexible Protein Regions on Target RNA Binding., Rao JN, Schweimer K, Wenzel S, Wohrl BM, Rosch P, Biochemistry. 2008 Mar 25;47(12):3756-61. Epub 2008 Feb 28. PMID:18303858

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