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6aeq

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Crystal structure of the ssDNA-binding domain of DnaT from Salmonella enterica Serovar Typhimurium LT2

Structural highlights

6aeq is a 2 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2518084Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DNAT_SALTY This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N a prepriming protein complex on the specific site of the template DNA recognized by protein N'.

Publication Abstract from PubMed

DnaT is a replication restart primosomal protein required for re-initiating chromosomal DNA replication in bacteria. DnaT can be a monomer, dimer, trimer, tetramer, or pentamer. The oligomerization and disassembly of DnaT oligomers are critical in primosome assembly. Prior to this work, only the ssDNA-bound structure of the pentameric DnaT truncated protein (aa 84-153; DnaT84-153) was available. The mechanism by which DnaT oligomerizes as different states is unclear. In this paper, we report the crystal structure of the C-terminal domain of DnaT (aa 84-179; DnaTc) at 2.30A resolution (PDB entry 6AEQ). DnaTc forms a dimer both in the crystalline state and in solution. As compared with the ssDNA-bound structure of the pentameric DnaT84-153, their subunit-subunit interfaces significantly differ. The different oligomeric architecture suggests a strong conformational change possibly induced by ssDNA. Superposition analysis further indicated that the monomer of a DnaTc dimer shifted away by a distance of 7.5A and rotated by an angle of 170 degrees for binding to ssDNA. Basing from these molecular evidence, we discussed and proposed a working model to explain how DnaTc oligomerizes through residue R146 mediation.

Crystal structure of the C-terminal domain of the primosomal DnaT protein: Insights into a new oligomerization mechanism.,Chen KL, Huang YH, Liao JF, Lee WC, Huang CY Biochem Biophys Res Commun. 2019 Mar 26;511(1):1-6. doi:, 10.1016/j.bbrc.2019.02.026. Epub 2019 Feb 10. PMID:30755302^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen KL, Huang YH, Liao JF, Lee WC, Huang CY. Crystal structure of the C-terminal domain of the primosomal DnaT protein: Insights into a new oligomerization mechanism. Biochem Biophys Res Commun. 2019 Mar 26;511(1):1-6. doi:, 10.1016/j.bbrc.2019.02.026. Epub 2019 Feb 10. PMID:30755302 doi:http://dx.doi.org/10.1016/j.bbrc.2019.02.026

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6aeq"

Categories: Large Structures | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 | Huang CY | Huang YH

@@ Line 3: / Line 3: @@
 <StructureSection load='6aeq' size='340' side='right'caption='[[6aeq]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6aeq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AEQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6aeq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AEQ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aeq OCA], [http://pdbe.org/6aeq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aeq RCSB], [http://www.ebi.ac.uk/pdbsum/6aeq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aeq ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2518084&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6aeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aeq OCA], [https://pdbe.org/6aeq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6aeq RCSB], [https://www.ebi.ac.uk/pdbsum/6aeq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6aeq ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/DNAT_SALTY DNAT_SALTY]] This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.
+[https://www.uniprot.org/uniprot/DNAT_SALTY DNAT_SALTY] This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Huang, C Y]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
-[[Category: Huang, Y H]]
+[[Category: Huang CY]]
-[[Category: Dna binding]]
+[[Category: Huang YH]]
-[[Category: Dna binding protein]]
-[[Category: Dnat]]
-[[Category: Primosome]]
-[[Category: Replication restart]]

6aeq

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Current revision

Crystal structure of the ssDNA-binding domain of DnaT from Salmonella enterica Serovar Typhimurium LT2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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