This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4cbu

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 17:37, 7 September 2022

Crystal structure of Plasmodium falciparum actin I

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4cbu"

@@ Line 3: / Line 3: @@
 <StructureSection load='4cbu' size='340' side='right'caption='[[4cbu]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4cbu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4cbu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CBU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cbw|4cbw]], [[4cbx|4cbx]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbu OCA], [https://pdbe.org/4cbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cbu RCSB], [https://www.ebi.ac.uk/pdbsum/4cbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbu ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbu OCA], [http://pdbe.org/4cbu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cbu RCSB], [http://www.ebi.ac.uk/pdbsum/4cbu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbu ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ACT1_PLAFX ACT1_PLAFX]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Actin assembles into short polymer microfilaments, these are thought to contribute to parasite gliding motility. [[http://www.uniprot.org/uniprot/GELS_MOUSE GELS_MOUSE]] Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
+[[https://www.uniprot.org/uniprot/ACT1_PLAFX ACT1_PLAFX]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Actin assembles into short polymer microfilaments, these are thought to contribute to parasite gliding motility.
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Actins are highly conserved proteins and key players in central processes in all eukaryotic cells. The two actins of the malaria parasite are among the most divergent eukaryotic actins and also differ from each other more than isoforms in any other species. Microfilaments have not been directly observed in Plasmodium and are presumed to be short and highly dynamic. We show that actin I cannot complement actin II in male gametogenesis, suggesting critical structural differences. Cryo-EM reveals that Plasmodium actin I has a unique filament structure, whereas actin II filaments resemble canonical F-actin. Both Plasmodium actins hydrolyze ATP more efficiently than alpha-actin, and unlike any other actin, both parasite actins rapidly form short oligomers induced by ADP. Crystal structures of both isoforms pinpoint several structural changes in the monomers causing the unique polymerization properties. Inserting the canonical D-loop to Plasmodium actin I leads to the formation of long filaments in vitro. In vivo, this chimera restores gametogenesis in parasites lacking actin II, suggesting that stable filaments are required for exflagellation. Together, these data underline the divergence of eukaryotic actins and demonstrate how structural differences in the monomers translate into filaments with different properties, implying that even eukaryotic actins have faced different evolutionary pressures and followed different paths for developing their polymerization properties.
-Structural differences explain diverse functions of Plasmodium actins.,Vahokoski J, Bhargav SP, Desfosses A, Andreadaki M, Kumpula EP, Martinez SM, Ignatev A, Lepper S, Frischknecht F, Siden-Kiamos I, Sachse C, Kursula I PLoS Pathog. 2014 Apr 17;10(4):e1004091. doi: 10.1371/journal.ppat.1004091., eCollection 2014 Apr. PMID:24743229<ref>PMID:24743229</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 4cbu" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Actin 3D structures|Actin 3D structures]]
-*[[Gelsolin|Gelsolin]]
+*[[Gelsolin 3D structures|Gelsolin 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Plafa]]
+[[Category: Plasmodium falciparum]]
-[[Category: Andreadaki, M]]
+[[Category: Andreadaki M]]
-[[Category: Bhargav, S P]]
+[[Category: Bhargav SP]]
-[[Category: Desfosses, A]]
+[[Category: Desfosses A]]
-[[Category: Frischknecht, F]]
+[[Category: Frischknecht F]]
-[[Category: Ignatev, A]]
+[[Category: Ignatev A]]
-[[Category: Kumpula, E P]]
+[[Category: Kumpula EP]]
-[[Category: Kursula, I]]
+[[Category: Kursula I]]
-[[Category: Lepper, S]]
+[[Category: Lepper S]]
-[[Category: Martinez, S Munico]]
+[[Category: Munico Martinez S]]
-[[Category: Sachse, C]]
+[[Category: Sachse C]]
-[[Category: Siden-Kiamos, I]]
+[[Category: Siden-Kiamos I]]
-[[Category: Vahokoski, J]]
+[[Category: Vahokoski J]]
-[[Category: Malaria]]
-[[Category: Motility]]
-[[Category: Motor protein]]
-[[Category: Parasite]]

4cbu

From Proteopedia

Revision as of 17:37, 7 September 2022

Crystal structure of Plasmodium falciparum actin I

Structural highlights

Function

See Also

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox