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| | <StructureSection load='4d0a' size='340' side='right'caption='[[4d0a]], [[Resolution|resolution]] 6.00Å' scene=''> | | <StructureSection load='4d0a' size='340' side='right'caption='[[4d0a]], [[Resolution|resolution]] 6.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4d0a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D0A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4d0a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D0A FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d0a OCA], [http://pdbe.org/4d0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d0a RCSB], [http://www.ebi.ac.uk/pdbsum/4d0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d0a ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d0a OCA], [https://pdbe.org/4d0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d0a RCSB], [https://www.ebi.ac.uk/pdbsum/4d0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d0a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NAH1_METJA NAH1_METJA]] This is a Na(+)/H(+) antiporter. Can also transport lithium.<ref>PMID:12220668</ref> | + | [[https://www.uniprot.org/uniprot/NAH1_METJA NAH1_METJA]] This is a Na(+)/H(+) antiporter. Can also transport lithium.<ref>PMID:12220668</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Metja]] | + | [[Category: Methanocaldococcus jannaschii DSM 2661]] |
| - | [[Category: Kuhlbrandt, W]] | + | [[Category: Kuhlbrandt W]] |
| - | [[Category: Paulino, C]] | + | [[Category: Paulino C]] |
| - | [[Category: Woehlert, D]] | + | [[Category: Woehlert D]] |
| - | [[Category: Yildiz, O]] | + | [[Category: Yildiz O]] |
| - | [[Category: Antiporter]]
| + | |
| - | [[Category: Cpa]]
| + | |
| - | [[Category: Exchanger]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Transporter]]
| + | |
| Structural highlights
Function
[NAH1_METJA] This is a Na(+)/H(+) antiporter. Can also transport lithium.[1]
Publication Abstract from PubMed
Sodium/proton antiporters are essential for sodium and pH homeostasis and play a major role in human health and disease. We determined the structures of the archaeal sodium/proton antiporter MjNhaP1 in two complementary states. The inward-open state was obtained by x-ray crystallography in the presence of sodium at pH8, where the transporter is highly active. The outward-open state was obtained by electron crystallography without sodium at pH4, where MjNhaP1 is inactive. Comparison of both structures reveals a 7{degree sign} tilt of the 6 helix bundle. 22Na+ uptake measurements indicate non-cooperative transport with an activity maximum at pH7.5. We conclude that binding of a Na+ ion from the outside induces helix movements that close the extracellular cavity, open the cytoplasmic funnel, and result in a ~5 A vertical relocation of the ion binding site to release the substrate ion into the cytoplasm.
Structure and transport mechanism of the sodium/protonantiporter MjNhaP1.,Paulino C, Wohlert D, Kapotova E, Yildiz O, Kuhlbrandt W Elife. 2014 Nov 26;3. doi: 10.7554/eLife.03583. PMID:25426803[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hellmer J, Patzold R, Zeilinger C. Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii. FEBS Lett. 2002 Sep 11;527(1-3):245-9. PMID:12220668
- ↑ Paulino C, Wohlert D, Kapotova E, Yildiz O, Kuhlbrandt W. Structure and transport mechanism of the sodium/protonantiporter MjNhaP1. Elife. 2014 Nov 26;3. doi: 10.7554/eLife.03583. PMID:25426803 doi:http://dx.doi.org/10.7554/eLife.03583
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