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4c98

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<StructureSection load='4c98' size='340' side='right'caption='[[4c98]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='4c98' size='340' side='right'caption='[[4c98]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4c98]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C98 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4c98]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C98 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c8y|4c8y]], [[4c8z|4c8z]], [[4c97|4c97]], [[4c9d|4c9d]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c98 OCA], [https://pdbe.org/4c98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c98 RCSB], [https://www.ebi.ac.uk/pdbsum/4c98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c98 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c98 OCA], [http://pdbe.org/4c98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c98 RCSB], [http://www.ebi.ac.uk/pdbsum/4c98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c98 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/Q53VU8_THET8 Q53VU8_THET8]]
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In many bacteria and archaea, small RNAs derived from clustered regularly interspaced short palindromic repeats (CRISPRs) associate with CRISPR-associated (Cas) proteins to target foreign DNA for destruction. In Type I and III CRISPR/Cas systems, the Cas6 family of endoribonucleases generates functional CRISPR-derived RNAs by site-specific cleavage of repeat sequences in precursor transcripts. CRISPR repeats differ widely in both sequence and structure, with varying propensity to form hairpin folds immediately preceding the cleavage site. To investigate the evolution of distinct mechanisms for the recognition of diverse CRISPR repeats by Cas6 enzymes, we determined crystal structures of two Thermus thermophilus Cas6 enzymes both alone and bound to substrate and product RNAs. These structures show how the scaffold common to all Cas6 endonucleases has evolved two binding sites with distinct modes of RNA recognition: one specific for a hairpin fold and the other for a single-stranded 5'-terminal segment preceding the hairpin. These findings explain how divergent Cas6 enzymes have emerged to mediate highly selective pre-CRISPR-derived RNA processing across diverse CRISPR systems.
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Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases.,Niewoehner O, Jinek M, Doudna JA Nucleic Acids Res. 2013 Oct 22. PMID:24150936<ref>PMID:24150936</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4c98" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[CRISPR subtype I-B|CRISPR subtype I-B]]
*[[CRISPR subtype I-B|CRISPR subtype I-B]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Thet8]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Doudna, J A]]
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[[Category: Doudna JA]]
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[[Category: Jinek, M]]
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[[Category: Jinek M]]
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[[Category: Niewoehner, O]]
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[[Category: Niewoehner O]]
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[[Category: Cas protein]]
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[[Category: Crispr]]
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[[Category: Hydrolase]]
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[[Category: Ribonuclease]]
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[[Category: Rna processing]]
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Revision as of 17:33, 7 September 2022

Thermus thermophilus Cas6 (TTHB231)

PDB ID 4c98

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