6gf7
From Proteopedia
(Difference between revisions)
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- | '''Unreleased structure''' | ||
- | + | ==Molecular basis of egg coat filament cross-linking: Zn-SAD structure of the partially deglycosylated ZP1 ZP-N1 domain homodimer== | |
+ | <StructureSection load='6gf7' size='340' side='right'caption='[[6gf7]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[6gf7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GF7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GF7 FirstGlance]. <br> | ||
+ | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
+ | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6gf6|6gf6]], [[6gf8|6gf8]]</td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gf7 OCA], [http://pdbe.org/6gf7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gf7 RCSB], [http://www.ebi.ac.uk/pdbsum/6gf7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gf7 ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | As the result of a combined biochemical and electron microscopic investigation, hitherto unrecognized structural features of the mouse egg extracellular coat, or zona pellucida, have been revealed. Specimens were prepared for electron microscopy by spraying individually isolated zonae pellucidae onto a substrate and were observed by both rotary shadowing and negative staining techniques. Results of these experiments suggest that the three zona pellucida glycoproteins, ZP1 (200,000 Mr), ZP2 (120,000 Mr) and ZP3 (83,000 Mr), are organized into long filaments. Negatively stained zona pellucida filaments resemble "beads-on-a-string", with each bead (9.5 nm in diameter) located every 17 nm or so (center-to-center distance) along the axis of the filament. The filaments, in turn, appear to be interconnected by one of the three zona pellucida glycoproteins, ZP1, giving rise to a three-dimensional matrix. Proteolysis of ZP1 by chymotrypsin or reduction of intermolecular disulfides of ZP1 by dithiothreitol results in both solubilization of zonae pellucidae and disruption of interconnections between individual zona pellucida filaments. These observations suggest that the zona pellucida, which plays important roles both during and after fertilization of mammalian eggs, is a highly organized extracellular coat in which glycoproteins are assembled into filaments possessing a recognizable structural repeat. | ||
- | + | Mouse egg extracellular coat is a matrix of interconnected filaments possessing a structural repeat.,Greve JM, Wassarman PM J Mol Biol. 1985 Jan 20;181(2):253-64. PMID:3845123<ref>PMID:3845123</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
+ | <div class="pdbe-citations 6gf7" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Jovine, L]] | ||
+ | [[Category: Nishimura, K]] | ||
+ | [[Category: Cell adhesion]] | ||
+ | [[Category: Egg coat filament cross-linking]] | ||
+ | [[Category: Egg coat penetration by sperm]] | ||
+ | [[Category: Zona pellucida]] | ||
+ | [[Category: Zp domain]] | ||
+ | [[Category: Zp module]] | ||
+ | [[Category: Zp-n domain]] | ||
+ | [[Category: Zp1]] |
Revision as of 05:52, 19 June 2019
Molecular basis of egg coat filament cross-linking: Zn-SAD structure of the partially deglycosylated ZP1 ZP-N1 domain homodimer
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