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5t4c

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Crystal structure of BhGH81 mutant in complex with laminaro-hexaose

Structural highlights

5t4c is a 1 chain structure with sequence from Alkalihalobacillus halodurans C-125. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENG1_HALH5 Cleaves internal linkages in 1,3-beta-glucan (PubMed:15501830, PubMed:28827308, PubMed:28781080). May contribute to plant biomass degradation (By similarity).[UniProtKB:Q47N06]^[1] ^[2] ^[3]

Publication Abstract from PubMed

Family 81 glycoside hydrolases (GHs), which are known to cleave beta-1,3-glucans, are found in archaea, bacteria, eukaryotes, and viruses. Here we examine the structural and functional features of the GH81 catalytic module, BhGH81, from the Bacillus halodurans protein BH0236 to probe the molecular basis of beta-1,3-glucan recognition and cleavage. BhGH81 displayed activity on laminarin, curdlan, and pachyman, but not scleroglucan; the enzyme also cleaved beta-1,3-glucooligosaccharides as small as beta-1,3-glucotriose. The crystal structures of BhGH81 in complex with various beta-1,3-glucooligosaccharides revealed distorted sugars in the -1 catalytic subsite and an arrangement consistent with an inverting catalytic mechanism having a proposed conformational itinerary of (2)S0 --> (2,5)B(double dagger) --> (5)S1. Notably, the architecture of the catalytic site, location of an adjacent ancillary beta-1,3-glucan binding site, and the surface properties of the enzyme indicate the likely ability to recognize the double and/or triple-helical quaternary structures adopted by beta-1,3-glucans.

Structural Analysis of a Family 81 Glycoside Hydrolase Implicates Its Recognition of beta-1,3-Glucan Quaternary Structure.,Pluvinage B, Fillo A, Massel P, Boraston AB Structure. 2017 Sep 5;25(9):1348-1359.e3. doi: 10.1016/j.str.2017.06.019. Epub, 2017 Aug 3. PMID:28781080^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ van Bueren AL, Morland C, Gilbert HJ, Boraston AB. Family 6 carbohydrate binding modules recognize the non-reducing end of beta-1,3-linked glucans by presenting a unique ligand binding surface. J Biol Chem. 2005 Jan 7;280(1):530-7. Epub 2004 Oct 22. PMID:15501830 doi:10.1074/jbc.M410113200
↑ Pluvinage B, Fillo A, Massel P, Boraston AB. Structural Analysis of a Family 81 Glycoside Hydrolase Implicates Its Recognition of beta-1,3-Glucan Quaternary Structure. Structure. 2017 Sep 5;25(9):1348-1359.e3. doi: 10.1016/j.str.2017.06.019. Epub, 2017 Aug 3. PMID:28781080 doi:http://dx.doi.org/10.1016/j.str.2017.06.019
↑ Hettle A, Fillo A, Abe K, Massel P, Pluvinage B, Langelaan DN, Smith SP, Boraston AB. Properties of a family 56 carbohydrate-binding module and its role in the recognition and hydrolysis of beta-1,3-glucan. J Biol Chem. 2017 Oct 13;292(41):16955-16968. doi: 10.1074/jbc.M117.806711. Epub , 2017 Aug 21. PMID:28827308 doi:http://dx.doi.org/10.1074/jbc.M117.806711
↑ Pluvinage B, Fillo A, Massel P, Boraston AB. Structural Analysis of a Family 81 Glycoside Hydrolase Implicates Its Recognition of beta-1,3-Glucan Quaternary Structure. Structure. 2017 Sep 5;25(9):1348-1359.e3. doi: 10.1016/j.str.2017.06.019. Epub, 2017 Aug 3. PMID:28781080 doi:http://dx.doi.org/10.1016/j.str.2017.06.019

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5t4c"

Categories: Alkalihalobacillus halodurans C-125 | Large Structures | Boraston AB | Pluvinage B

@@ Line 3: / Line 3: @@
 <StructureSection load='5t4c' size='340' side='right'caption='[[5t4c]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5t4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bachd Bachd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T4C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5t4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_halodurans_C-125 Alkalihalobacillus halodurans C-125]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T4C FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5t49|5t49]], [[5t4a|5t4a]], [[5t4g|5t4g]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BH0236 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272558 BACHD])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t4c OCA], [https://pdbe.org/5t4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t4c RCSB], [https://www.ebi.ac.uk/pdbsum/5t4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t4c ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t4c OCA], [http://pdbe.org/5t4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t4c RCSB], [http://www.ebi.ac.uk/pdbsum/5t4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t4c ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/ENG1_HALH5 ENG1_HALH5] Cleaves internal linkages in 1,3-beta-glucan (PubMed:15501830, PubMed:28827308, PubMed:28781080). May contribute to plant biomass degradation (By similarity).[UniProtKB:Q47N06]<ref>PMID:15501830</ref> <ref>PMID:28781080</ref> <ref>PMID:28827308</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Bachd]]
+[[Category: Alkalihalobacillus halodurans C-125]]
 [[Category: Large Structures]]
-[[Category: Boraston, A B]]
+[[Category: Boraston AB]]
-[[Category: Pluvinage, B]]
+[[Category: Pluvinage B]]
-[[Category: Glycoside hydrolase]]
-[[Category: Hydrolase]]

5t4c

From Proteopedia

Current revision

Crystal structure of BhGH81 mutant in complex with laminaro-hexaose

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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