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| | <StructureSection load='4ewc' size='340' side='right'caption='[[4ewc]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='4ewc' size='340' side='right'caption='[[4ewc]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ewc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Isav Isav]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EWC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EWC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ewc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmon_isavirus Salmon isavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EWC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EWC FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ewc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ewc OCA], [http://pdbe.org/4ewc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ewc RCSB], [http://www.ebi.ac.uk/pdbsum/4ewc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ewc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ewc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ewc OCA], [https://pdbe.org/4ewc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ewc RCSB], [https://www.ebi.ac.uk/pdbsum/4ewc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ewc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/NCAP_ISAV8 NCAP_ISAV8] Encapsidates the negative strand viral RNA, protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication (PubMed:15522447).<ref>PMID:15522447</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Isav]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tao, Y J]] | + | [[Category: Salmon isavirus]] |
| - | [[Category: Zheng, W]] | + | [[Category: Tao YJ]] |
| - | [[Category: Alpha-helical]] | + | [[Category: Zheng W]] |
| - | [[Category: Isavirus]]
| + | |
| - | [[Category: Np]]
| + | |
| - | [[Category: Nucleoprotein]]
| + | |
| - | [[Category: Orthomyoxovirus]]
| + | |
| - | [[Category: Replication]]
| + | |
| - | [[Category: Rna binding]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna encapsidation]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Viral rna packaging]]
| + | |
| Structural highlights
Function
NCAP_ISAV8 Encapsidates the negative strand viral RNA, protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication (PubMed:15522447).[1]
Publication Abstract from PubMed
Genome packaging for viruses with segmented genomes is often a complex problem. This is particularly true for influenza viruses and other orthomyxoviruses, whose genome consists of multiple negative-sense RNAs encapsidated as ribonucleoprotein (RNP) complexes. To better understand the structural features of orthomyxovirus RNPs that allow them to be packaged, we determined the crystal structure of the nucleoprotein (NP) of a fish orthomyxovirus, the infectious salmon anemia virus (ISAV) (genus Isavirus). As the major protein component of the RNPs, ISAV-NP possesses a bi-lobular structure similar to the influenza virus NP. Because both RNA-free and RNA-bound ISAV NP forms stable dimers in solution, we were able to measure the NP RNA binding affinity as well as the stoichiometry using recombinant proteins and synthetic oligos. Our RNA binding analysis revealed that each ISAV-NP binds ~12 nts of RNA, shorter than the 24-28 nts originally estimated for the influenza A virus NP based on population average. The 12-nt stoichiometry was further confirmed by results from electron microscopy and dynamic light scattering. Considering that RNPs of ISAV and the influenza viruses have similar morphologies and dimensions, our findings suggest that NP-free RNA may exist on orthomyxovirus RNPs, and selective RNP packaging may be accomplished through direct RNA-RNA interactions.
The crystal structure and RNA-binding of an orthomyxovirus nucleoprotein.,Zheng W, Olson J, Vakharia V, Tao YJ PLoS Pathog. 2013 Sep;9(9):e1003624. doi: 10.1371/journal.ppat.1003624. Epub 2013, Sep 12. PMID:24068932[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Aspehaug V, Falk K, Krossoy B, Thevarajan J, Sanders L, Moore L, Endresen C, Biering E. Infectious salmon anemia virus (ISAV) genomic segment 3 encodes the viral nucleoprotein (NP), an RNA-binding protein with two monopartite nuclear localization signals (NLS). Virus Res. 2004 Nov;106(1):51-60. doi: 10.1016/j.virusres.2004.06.001. PMID:15522447 doi:http://dx.doi.org/10.1016/j.virusres.2004.06.001
- ↑ Zheng W, Olson J, Vakharia V, Tao YJ. The crystal structure and RNA-binding of an orthomyxovirus nucleoprotein. PLoS Pathog. 2013 Sep;9(9):e1003624. doi: 10.1371/journal.ppat.1003624. Epub 2013, Sep 12. PMID:24068932 doi:http://dx.doi.org/10.1371/journal.ppat.1003624
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