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| | <StructureSection load='4d6c' size='340' side='right'caption='[[4d6c]], [[Resolution|resolution]] 1.59Å' scene=''> | | <StructureSection load='4d6c' size='340' side='right'caption='[[4d6c]], [[Resolution|resolution]] 1.59Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4d6c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae_sp3-bs71 Streptococcus pneumoniae sp3-bs71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D6C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4d6c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_SP3-BS71 Streptococcus pneumoniae SP3-BS71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D6C FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4d6d|4d6d]], [[4d6e|4d6e]], [[4d6f|4d6f]], [[4d6g|4d6g]], [[4d6h|4d6h]], [[4d6i|4d6i]], [[4d6j|4d6j]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d6c OCA], [https://pdbe.org/4d6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d6c RCSB], [https://www.ebi.ac.uk/pdbsum/4d6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d6c ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Blood-group-substance_endo-1,4-beta-galactosidase Blood-group-substance endo-1,4-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.102 3.2.1.102] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d6c OCA], [http://pdbe.org/4d6c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d6c RCSB], [http://www.ebi.ac.uk/pdbsum/4d6c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d6c ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Blood-group-substance endo-1,4-beta-galactosidase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Streptococcus pneumoniae sp3-bs71]] | + | [[Category: Streptococcus pneumoniae SP3-BS71]] |
| - | [[Category: Boraston, A B]] | + | [[Category: Boraston AB]] |
| - | [[Category: Chapanian, R]] | + | [[Category: Chapanian R]] |
| - | [[Category: Constantinescu, I]] | + | [[Category: Constantinescu I]] |
| - | [[Category: Higgins, M A]] | + | [[Category: Higgins MA]] |
| - | [[Category: Kizhakkedathu, J N]] | + | [[Category: Kizhakkedathu JN]] |
| - | [[Category: Kwan, D H]] | + | [[Category: Kwan DH]] |
| - | [[Category: Samain, E]] | + | [[Category: Samain E]] |
| - | [[Category: Withers, S G]] | + | [[Category: Withers SG]] |
| - | [[Category: Blood group antigen]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Blood transfusions are critically important in many medical procedures, but the presence of antigens on red blood cells (RBCs, erythrocytes) means that careful blood-typing must be carried out prior to transfusion to avoid adverse and sometimes fatal reactions following transfusion. Enzymatic removal of the terminal N-acetylgalactosamine or galactose of A- or B-antigens, respectively, yields universal O-type blood, but is inefficient. Starting with the family 98 glycoside hydrolase from Streptococcus pneumoniae SP3-BS71 (Sp3GH98), which cleaves the entire terminal trisaccharide antigenic determinants of both A- and B-antigens from some of the linkages on RBC surface glycans, through several rounds of evolution, we developed variants with vastly improved activity toward some of the linkages that are resistant to cleavage by the wild-type enzyme. The resulting enzyme effects more complete removal of blood group antigens from cell surfaces, demonstrating the potential for engineering enzymes to generate antigen-null blood from donors of various types.
Toward Efficient Enzymes for the Generation of Universal Blood through Structure-Guided Directed Evolution.,Kwan DH, Constantinescu I, Chapanian R, Higgins MA, Kotzler MP, Samain E, Boraston AB, Kizhakkedathu JN, Withers SG J Am Chem Soc. 2015 Apr 24. PMID:25870881[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kwan DH, Constantinescu I, Chapanian R, Higgins MA, Kotzler MP, Samain E, Boraston AB, Kizhakkedathu JN, Withers SG. Toward Efficient Enzymes for the Generation of Universal Blood through Structure-Guided Directed Evolution. J Am Chem Soc. 2015 Apr 24. PMID:25870881 doi:http://dx.doi.org/10.1021/ja5116088
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