| Structural highlights
6nuw is a 13 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | NonStd Res: | |
| Gene: | IML3, MCM19, YBR107C, YBR0836 (Baker's yeast), MCM21, CTF5, YDR318W (Baker's yeast), CTF19, MCM18, YPL018W, LPB13W (Baker's yeast), CHL4, CTF17, MCM17, YDR254W, YD9320A.04 (Baker's yeast), OKP1, YGR179C (Baker's yeast), NKP1, YDR383C (Baker's yeast), AME1, ARP100, YBR211C, YBR1458 (Baker's yeast), CTF3, CHL3, YLR381W (Baker's yeast), NKP2, YLR315W (Baker's yeast) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[NKP1_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[1] [CENPP_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[2] [3] [CENPU_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[4] [NKP2_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[5] [CENPO_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[6] [CENPI_YEAST] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[7] [CENPL_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[8] [9] [CENPN_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[10] [CENPQ_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.[11] [12]
Publication Abstract from PubMed
Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) in other organisms creates the foundation of a kinetochore. The Ctf19c/CCAN influences the timing of kinetochore assembly, sets its location by associating with a specialized nucleosome containing the histone H3 variant Cse4/CENP-A, and determines the organization of the microtubule attachment apparatus. We present here the structure of a reconstituted 13-subunit Ctf19c determined by cryo-electron microscopy at ~4 A resolution. The structure accounts for known and inferred contacts with the Cse4 nucleosome and for an observed assembly hierarchy. We describe its implications for establishment of kinetochores and for their regulation by kinases throughout the cell cycle.
The structure of the Ctf19c/CCAN from budding yeast.,Hinshaw SM, Harrison SC Elife. 2019 Feb 14;8. pii: 44239. doi: 10.7554/eLife.44239. PMID:30762520[13]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schleiffer A, Maier M, Litos G, Lampert F, Hornung P, Mechtler K, Westermann S. CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol. 2012 May 6;14(6):604-13. doi: 10.1038/ncb2493. PMID:22561346 doi:http://dx.doi.org/10.1038/ncb2493
- ↑ Ortiz J, Stemmann O, Rank S, Lechner J. A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev. 1999 May 1;13(9):1140-55. PMID:10323865
- ↑ De Wulf P, McAinsh AD, Sorger PK. Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 2003 Dec 1;17(23):2902-21. Epub 2003 Nov 21. PMID:14633972 doi:http://dx.doi.org/10.1101/gad.1144403
- ↑ De Wulf P, McAinsh AD, Sorger PK. Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 2003 Dec 1;17(23):2902-21. Epub 2003 Nov 21. PMID:14633972 doi:http://dx.doi.org/10.1101/gad.1144403
- ↑ Schleiffer A, Maier M, Litos G, Lampert F, Hornung P, Mechtler K, Westermann S. CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol. 2012 May 6;14(6):604-13. doi: 10.1038/ncb2493. PMID:22561346 doi:http://dx.doi.org/10.1038/ncb2493
- ↑ Poddar A, Roy N, Sinha P. MCM21 and MCM22, two novel genes of the yeast Saccharomyces cerevisiae are required for chromosome transmission. Mol Microbiol. 1999 Jan;31(1):349-60. PMID:9987135
- ↑ Cheeseman IM, Anderson S, Jwa M, Green EM, Kang J, Yates JR 3rd, Chan CS, Drubin DG, Barnes G. Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell. 2002 Oct 18;111(2):163-72. PMID:12408861
- ↑ Ghosh SK, Poddar A, Hajra S, Sanyal K, Sinha P. The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation. Mol Genet Genomics. 2001 Apr;265(2):249-57. PMID:11361335
- ↑ Ghosh SK, Sau S, Lahiri S, Lohia A, Sinha P. The Iml3 protein of the budding yeast is required for the prevention of precocious sister chromatid separation in meiosis I and for sister chromatid disjunction in meiosis II. Curr Genet. 2004 Aug;46(2):82-91. Epub 2004 Jul 6. PMID:15241623 doi:http://dx.doi.org/10.1007/s00294-004-0516-6
- ↑ Roy N, Poddar A, Lohia A, Sinha P. The mcm17 mutation of yeast shows a size-dependent segregational defect of a mini-chromosome. Curr Genet. 1997 Sep;32(3):182-9. PMID:9339342
- ↑ Ortiz J, Stemmann O, Rank S, Lechner J. A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev. 1999 May 1;13(9):1140-55. PMID:10323865
- ↑ De Wulf P, McAinsh AD, Sorger PK. Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 2003 Dec 1;17(23):2902-21. Epub 2003 Nov 21. PMID:14633972 doi:http://dx.doi.org/10.1101/gad.1144403
- ↑ Hinshaw SM, Harrison SC. The structure of the Ctf19c/CCAN from budding yeast. Elife. 2019 Feb 14;8. pii: 44239. doi: 10.7554/eLife.44239. PMID:30762520 doi:http://dx.doi.org/10.7554/eLife.44239
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