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2jki

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Revision as of 11:27, 31 March 2021

Complex of Hsp90 N-terminal and Sgt1 CS domain

Structural highlights

2jki is a 6 chain structure with sequence from Arath and Barley. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SGT1A_ARATH] Functions in R gene-mediated resistance, but participates in a lower extent than SGT1B to RPP5-mediated resistance. Not required for RPM1, RPS2, RPS4 and RPS5-mediated resistance. Probably required for SCF-mediated ubiquitination, by coupling HSP90 to SCF complex for ubiquitination of HSP90 client proteins.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Sgt1 is an adaptor protein implicated in a variety of processes, including formation of the kinetochore complex in yeast, and regulation of innate immunity systems in plants and animals. Sgt1 has been found to associate with SCF E3 ubiquitin ligases, the CBF3 kinetochore complex, plant R proteins and related animal Nod-like receptors, and with the Hsp90 molecular chaperone. We have determined the crystal structure of the core Hsp90-Sgt1 complex, revealing a distinct site of interaction on the Hsp90 N-terminal domain. Using the structure, we developed mutations in Sgt1 interfacial residues, which specifically abrogate interaction with Hsp90, and disrupt Sgt1-dependent functions in vivo, in plants and yeast. We show that Sgt1 bridges the Hsp90 molecular chaperone system to the substrate-specific arm of SCF ubiquitin ligase complexes, suggesting a role in SCF assembly and regulation, and providing multiple complementary routes for ubiquitination of Hsp90 client proteins.

Structural and functional coupling of Hsp90- and Sgt1-centred multi-protein complexes.,Zhang M, Boter M, Li K, Kadota Y, Panaretou B, Prodromou C, Shirasu K, Pearl LH EMBO J. 2008 Oct 22;27(20):2789-98. Epub 2008 Sep 25. PMID:18818696^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Azevedo C, Betsuyaku S, Peart J, Takahashi A, Noel L, Sadanandom A, Casais C, Parker J, Shirasu K. Role of SGT1 in resistance protein accumulation in plant immunity. EMBO J. 2006 May 3;25(9):2007-16. Epub 2006 Apr 13. PMID:16619029 doi:http://dx.doi.org/10.1038/sj.emboj.7601084
↑ Zhang M, Boter M, Li K, Kadota Y, Panaretou B, Prodromou C, Shirasu K, Pearl LH. Structural and functional coupling of Hsp90- and Sgt1-centred multi-protein complexes. EMBO J. 2008 Oct 22;27(20):2789-98. Epub 2008 Sep 25. PMID:18818696 doi:10.1038/emboj.2008.190

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jki"

@@ Line 3: / Line 3: @@
 <StructureSection load='2jki' size='340' side='right'caption='[[2jki]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jki]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath] and [http://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JKI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jki]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath] and [https://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JKI FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jki OCA], [http://pdbe.org/2jki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jki RCSB], [http://www.ebi.ac.uk/pdbsum/2jki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jki ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jki OCA], [https://pdbe.org/2jki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jki RCSB], [https://www.ebi.ac.uk/pdbsum/2jki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jki ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SGT1A_ARATH SGT1A_ARATH]] Functions in R gene-mediated resistance, but participates in a lower extent than SGT1B to RPP5-mediated resistance. Not required for RPM1, RPS2, RPS4 and RPS5-mediated resistance. Probably required for SCF-mediated ubiquitination, by coupling HSP90 to SCF complex for ubiquitination of HSP90 client proteins.<ref>PMID:16619029</ref>
+[[https://www.uniprot.org/uniprot/SGT1A_ARATH SGT1A_ARATH]] Functions in R gene-mediated resistance, but participates in a lower extent than SGT1B to RPP5-mediated resistance. Not required for RPM1, RPS2, RPS4 and RPS5-mediated resistance. Probably required for SCF-mediated ubiquitination, by coupling HSP90 to SCF complex for ubiquitination of HSP90 client proteins.<ref>PMID:16619029</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Heat Shock Proteins|Heat Shock Proteins]]
+*[[Heat Shock Protein structures|Heat Shock Protein structures]]
 == References ==
 <references/>

2jki

From Proteopedia

Revision as of 11:27, 31 March 2021

Complex of Hsp90 N-terminal and Sgt1 CS domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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