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| | <StructureSection load='5amr' size='340' side='right'caption='[[5amr]], [[Resolution|resolution]] 2.57Å' scene=''> | | <StructureSection load='5amr' size='340' side='right'caption='[[5amr]], [[Resolution|resolution]] 2.57Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5amr]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bunlc Bunlc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AMR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5amr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/La_Crosse_virus La Crosse virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AMR FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5amq|5amq]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5amr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5amr OCA], [https://pdbe.org/5amr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5amr RCSB], [https://www.ebi.ac.uk/pdbsum/5amr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5amr ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5amr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5amr OCA], [http://pdbe.org/5amr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5amr RCSB], [http://www.ebi.ac.uk/pdbsum/5amr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5amr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/L_BUNLC L_BUNLC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[RNA polymerase|RNA polymerase]] | + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bunlc]] | + | [[Category: La Crosse virus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: RNA-directed RNA polymerase]]
| + | [[Category: Cusack S]] |
| - | [[Category: Cusack, S]] | + | [[Category: Gerlach P]] |
| - | [[Category: Gerlach, P]] | + | [[Category: Reguera J]] |
| - | [[Category: Reguera, J]] | + | |
| - | [[Category: Bunyavirus]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Polymerase]]
| + | |
| - | [[Category: Rna]]
| + | |
| - | [[Category: Rnadrnapol]]
| + | |
| Structural highlights
Function
L_BUNLC
Publication Abstract from PubMed
Segmented negative-strand RNA virus (sNSV) polymerases transcribe and replicate the viral RNA (vRNA) within a ribonucleoprotein particle (RNP). We present cryo-EM and X-ray structures of, respectively, apo- and vRNA bound La Crosse orthobunyavirus (LACV) polymerase that give atomic-resolution insight into how such RNPs perform RNA synthesis. The complementary 3' and 5' vRNA extremities are sequence specifically bound in separate sites on the polymerase. The 5' end binds as a stem-loop, allosterically structuring functionally important polymerase active site loops. Identification of distinct template and product exit tunnels allows proposal of a detailed model for template-directed replication with minimal disruption to the circularised RNP. The similar overall architecture and vRNA binding of monomeric LACV to heterotrimeric influenza polymerase, despite high sequence divergence, suggests that all sNSV polymerases have a common evolutionary origin and mechanism of RNA synthesis. These results will aid development of replication inhibitors of diverse, serious human pathogenic viruses.
Structural Insights into Bunyavirus Replication and Its Regulation by the vRNA Promoter.,Gerlach P, Malet H, Cusack S, Reguera J Cell. 2015 May 20. pii: S0092-8674(15)00553-X. doi: 10.1016/j.cell.2015.05.006. PMID:26004069[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Gerlach P, Malet H, Cusack S, Reguera J. Structural Insights into Bunyavirus Replication and Its Regulation by the vRNA Promoter. Cell. 2015 May 20. pii: S0092-8674(15)00553-X. doi: 10.1016/j.cell.2015.05.006. PMID:26004069 doi:http://dx.doi.org/10.1016/j.cell.2015.05.006
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