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| | <StructureSection load='5g47' size='340' side='right'caption='[[5g47]], [[Resolution|resolution]] 2.45Å' scene=''> | | <StructureSection load='5g47' size='340' side='right'caption='[[5g47]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5g47]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sfts_bunyavirus Sfts bunyavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G47 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5g47]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_fever_with_thrombocytopenia_syndrome_virus Severe fever with thrombocytopenia syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G47 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g47 OCA], [http://pdbe.org/5g47 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g47 RCSB], [http://www.ebi.ac.uk/pdbsum/5g47 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g47 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g47 OCA], [https://pdbe.org/5g47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g47 RCSB], [https://www.ebi.ac.uk/pdbsum/5g47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g47 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GP_SFTS GP_SFTS] Structural component of the virion that interacts with glycoprotein C (By similarity). It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion (By similarity). The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (By similarity). They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after clathrin-mediated endocytosis of the virion (PubMed:23388721). Plays a role in the packaging of ribonucleoproteins during virus assembly (By similarity).[UniProtKB:P09613][UniProtKB:P21401]<ref>PMID:23388721</ref> Structural component of the virion that interacts with glycoprotein N (By similarity). Acts as a class II fusion protein that is activated upon acidification and subsequent repositioning of the glycoprotein N (By similarity). The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (By similarity). They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after clathrin-mediated endocytosis of the virion (PubMed:23388721).[UniProtKB:P09613][UniProtKB:P21401]<ref>PMID:23388721</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 24: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sfts bunyavirus]] | + | [[Category: Severe fever with thrombocytopenia syndrome virus]] |
| - | [[Category: Behrens, A J]] | + | [[Category: Behrens AJ]] |
| - | [[Category: Bowden, T A]] | + | [[Category: Bowden TA]] |
| - | [[Category: Brennan, B]] | + | [[Category: Brennan B]] |
| - | [[Category: Crispin, M]] | + | [[Category: Crispin M]] |
| - | [[Category: Elliott, R M]] | + | [[Category: Elliott RM]] |
| - | [[Category: Halldorsson, S]] | + | [[Category: Halldorsson S]] |
| - | [[Category: Harlos, K]] | + | [[Category: Harlos K]] |
| - | [[Category: Huiskonen, J T]] | + | [[Category: Huiskonen JT]] |
| - | [[Category: Bunyavirus]]
| + | |
| - | [[Category: Class ii viral fusion]]
| + | |
| - | [[Category: Emerging virus]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Huaiyangshan virus]]
| + | |
| - | [[Category: Phlebovirus]]
| + | |
| - | [[Category: Viral membrane fusion]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| - | [[Category: Zoonosis]]
| + | |
| Structural highlights
Function
GP_SFTS Structural component of the virion that interacts with glycoprotein C (By similarity). It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion (By similarity). The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (By similarity). They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after clathrin-mediated endocytosis of the virion (PubMed:23388721). Plays a role in the packaging of ribonucleoproteins during virus assembly (By similarity).[UniProtKB:P09613][UniProtKB:P21401][1] Structural component of the virion that interacts with glycoprotein N (By similarity). Acts as a class II fusion protein that is activated upon acidification and subsequent repositioning of the glycoprotein N (By similarity). The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (By similarity). They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after clathrin-mediated endocytosis of the virion (PubMed:23388721).[UniProtKB:P09613][UniProtKB:P21401][2]
Publication Abstract from PubMed
An emergent viral pathogen termed severe fever with thrombocytopenia syndrome virus (SFTSV) is responsible for thousands of clinical cases and associated fatalities in China, Japan, and South Korea. Akin to other phleboviruses, SFTSV relies on a viral glycoprotein, Gc, to catalyze the merger of endosomal host and viral membranes during cell entry. Here, we describe the postfusion structure of SFTSV Gc, revealing that the molecular transformations the phleboviral Gc undergoes upon host cell entry are conserved with otherwise unrelated alpha- and flaviviruses. By comparison of SFTSV Gc with that of the prefusion structure of the related Rift Valley fever virus, we show that these changes involve refolding of the protein into a trimeric state. Reverse genetics and rescue of site-directed histidine mutants enabled localization of histidines likely to be important for triggering this pH-dependent process. These data provide structural and functional evidence that the mechanism of phlebovirus-host cell fusion is conserved among genetically and patho-physiologically distinct viral pathogens.
Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion.,Halldorsson S, Behrens AJ, Harlos K, Huiskonen JT, Elliott RM, Crispin M, Brennan B, Bowden TA Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7154-9. doi:, 10.1073/pnas.1603827113. Epub 2016 Jun 20. PMID:27325770[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hofmann H, Li X, Zhang X, Liu W, Kühl A, Kaup F, Soldan SS, González-Scarano F, Weber F, He Y, Pöhlmann S. Severe fever with thrombocytopenia virus glycoproteins are targeted by neutralizing antibodies and can use DC-SIGN as a receptor for pH-dependent entry into human and animal cell lines. J Virol. 2013 Apr;87(8):4384-94. PMID:23388721 doi:10.1128/JVI.02628-12
- ↑ Hofmann H, Li X, Zhang X, Liu W, Kühl A, Kaup F, Soldan SS, González-Scarano F, Weber F, He Y, Pöhlmann S. Severe fever with thrombocytopenia virus glycoproteins are targeted by neutralizing antibodies and can use DC-SIGN as a receptor for pH-dependent entry into human and animal cell lines. J Virol. 2013 Apr;87(8):4384-94. PMID:23388721 doi:10.1128/JVI.02628-12
- ↑ Halldorsson S, Behrens AJ, Harlos K, Huiskonen JT, Elliott RM, Crispin M, Brennan B, Bowden TA. Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion. Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7154-9. doi:, 10.1073/pnas.1603827113. Epub 2016 Jun 20. PMID:27325770 doi:http://dx.doi.org/10.1073/pnas.1603827113
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