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| | <StructureSection load='5j69' size='340' side='right'caption='[[5j69]], [[Resolution|resolution]] 3.63Å' scene=''> | | <StructureSection load='5j69' size='340' side='right'caption='[[5j69]], [[Resolution|resolution]] 3.63Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5j69]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J69 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5j69]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J69 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.63Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ASTN2, KIAA0634 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j69 OCA], [http://pdbe.org/5j69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j69 RCSB], [http://www.ebi.ac.uk/pdbsum/5j69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j69 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j69 OCA], [https://pdbe.org/5j69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j69 RCSB], [https://www.ebi.ac.uk/pdbsum/5j69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j69 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ASTN2_HUMAN ASTN2_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gilbert, R J.C]] | + | [[Category: Gilbert RJC]] |
| - | [[Category: Harlos, K]] | + | [[Category: Harlos K]] |
| - | [[Category: Ni, T]] | + | [[Category: Ni T]] |
| - | [[Category: Annexin-like domain]]
| + | |
| - | [[Category: Fibronectin]]
| + | |
| - | [[Category: Macpf domain]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Neural guidance]]
| + | |
| Structural highlights
Function
ASTN2_HUMAN
Publication Abstract from PubMed
The vertebrate-specific proteins astrotactin-1 and 2 (ASTN-1 and ASTN-2) are integral membrane perforin-like proteins known to play critical roles in neurodevelopment, while ASTN-2 has been linked to the planar cell polarity pathway in hair cells. Genetic variations associated with them are linked to a variety of neurodevelopmental disorders and other neurological pathologies, including an advanced onset of Alzheimer's disease. Here we present the structure of the majority endosomal region of ASTN-2, showing it to consist of a unique combination of polypeptide folds: a perforin-like domain, a minimal epidermal growth factor-like module, a unique form of fibronectin type III domain and an annexin-like domain. The perforin-like domain differs from that of other members of the membrane attack complex-perforin (MACPF) protein family in ways that suggest ASTN-2 does not form pores. Structural and biophysical data show that ASTN-2 (but not ASTN-1) binds inositol triphosphates, suggesting a mechanism for membrane recognition or secondary messenger regulation of its activity. The annexin-like domain is closest in fold to repeat three of human annexin V and similarly binds calcium, and yet shares no sequence homology with it. Overall, our structure provides the first atomic-resolution description of a MACPF protein involved in development, while highlighting distinctive features of ASTN-2 responsible for its activity.
Structure of astrotactin-2: a conserved vertebrate-specific and perforin-like membrane protein involved in neuronal development.,Ni T, Harlos K, Gilbert R Open Biol. 2016 May;6(5). pii: 160053. doi: 10.1098/rsob.160053. Epub 2016 May 4. PMID:27249642[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ni T, Harlos K, Gilbert R. Structure of astrotactin-2: a conserved vertebrate-specific and perforin-like membrane protein involved in neuronal development. Open Biol. 2016 May;6(5). pii: 160053. doi: 10.1098/rsob.160053. Epub 2016 May 4. PMID:27249642 doi:http://dx.doi.org/10.1098/rsob.160053
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