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| | <StructureSection load='4m1p' size='340' side='right'caption='[[4m1p]], [[Resolution|resolution]] 2.56Å' scene=''> | | <StructureSection load='4m1p' size='340' side='right'caption='[[4m1p]], [[Resolution|resolution]] 2.56Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4m1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geotn Geotn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M1P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4m1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermodenitrificans_NG80-2 Geobacillus thermodenitrificans NG80-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M1P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4adz|4adz]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m1p OCA], [https://pdbe.org/4m1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m1p RCSB], [https://www.ebi.ac.uk/pdbsum/4m1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m1p ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTNG_1533 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420246 GEOTN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m1p OCA], [http://pdbe.org/4m1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m1p RCSB], [http://www.ebi.ac.uk/pdbsum/4m1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m1p ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A4INJ9_GEOTN A4INJ9_GEOTN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Geotn]] | + | [[Category: Geobacillus thermodenitrificans NG80-2]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chang, F]] | + | [[Category: Chang F]] |
| - | [[Category: Giedroc, D P]] | + | [[Category: Dann III CE]] |
| - | [[Category: III, C E.Dann]]
| + | [[Category: Giedroc DP]] |
| - | [[Category: Transcription repressor]] | + | |
| Structural highlights
Function
A4INJ9_GEOTN
Publication Abstract from PubMed
The copper-sensing operon repressor (CsoR) is representative of a major Cu(I)-sensing family of bacterial metalloregulatory proteins that has evolved to prevent cytoplasmic copper toxicity. It is unknown how Cu(I) binding to tetrameric CsoRs mediates transcriptional derepression of copper resistance genes. A phylogenetic analysis of 227 DUF156 protein members, including biochemically or structurally characterized CsoR/RcnR repressors, reveals that Geobacillus thermodenitrificans (Gt) CsoR characterized here is representative of CsoRs from pathogenic bacilli Listeria monocytogenes and Bacillus anthracis. The 2.56 A structure of Cu(I)-bound Gt CsoR reveals that Cu(I) binding induces a kink in the alpha2-helix between two conserved copper-ligating residues and folds an N-terminal tail (residues 12-19) over the Cu(I) binding site. NMR studies of Gt CsoR reveal that this tail is flexible in the apo-state with these dynamics quenched upon Cu(I) binding. Small angle x-ray scattering experiments on an N-terminally truncated Gt CsoR (Delta2-10) reveal that the Cu(I)-bound tetramer is hydrodynamically more compact than is the apo-state. The implications of these findings for the allosteric mechanisms of other CsoR/RcnR repressors are discussed.
Cu(I)-mediated allosteric switching in a copper-sensing operon repressor (CsoR).,Chang FM, Coyne HJ, Cubillas C, Vinuesa P, Fang X, Ma Z, Ma D, Helmann JD, Garcia-de los Santos A, Wang YX, Dann CE 3rd, Giedroc DP J Biol Chem. 2014 Jul 4;289(27):19204-17. doi: 10.1074/jbc.M114.556704. Epub 2014, May 15. PMID:24831014[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chang FM, Coyne HJ, Cubillas C, Vinuesa P, Fang X, Ma Z, Ma D, Helmann JD, Garcia-de los Santos A, Wang YX, Dann CE 3rd, Giedroc DP. Cu(I)-mediated allosteric switching in a copper-sensing operon repressor (CsoR). J Biol Chem. 2014 Jul 4;289(27):19204-17. doi: 10.1074/jbc.M114.556704. Epub 2014, May 15. PMID:24831014 doi:http://dx.doi.org/10.1074/jbc.M114.556704
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