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| | <StructureSection load='4k8o' size='340' side='right'caption='[[4k8o]], [[Resolution|resolution]] 2.65Å' scene=''> | | <StructureSection load='4k8o' size='340' side='right'caption='[[4k8o]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4k8o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K8O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4k8o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K8O FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Abcb2, Mtp1, Tap1, Tap1 Abcb2 Mtp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k8o OCA], [https://pdbe.org/4k8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k8o RCSB], [https://www.ebi.ac.uk/pdbsum/4k8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k8o ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k8o OCA], [http://pdbe.org/4k8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k8o RCSB], [http://www.ebi.ac.uk/pdbsum/4k8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k8o ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TAP1_RAT TAP1_RAT]] Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity). | + | [https://www.uniprot.org/uniprot/TAP1_RAT TAP1_RAT] Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Abele, R]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Gaudet, R]] | + | [[Category: Abele R]] |
| - | [[Category: Grossmann, N]] | + | [[Category: Gaudet R]] |
| - | [[Category: Hulpke, S]] | + | [[Category: Grossmann N]] |
| - | [[Category: Tampe, R]] | + | [[Category: Hulpke S]] |
| - | [[Category: Vakkasoglu, A S]] | + | [[Category: Tampe R]] |
| - | [[Category: Nucleotide binding domain]]
| + | [[Category: Vakkasoglu AS]] |
| - | [[Category: Peptide transport]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
TAP1_RAT Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).
Publication Abstract from PubMed
The ATP-binding cassette (ABC) transporter associated with antigen processing (TAP) participates in immune surveillance by moving proteasomal products into the endoplasmic reticulum (ER) lumen for major histocompatibility complex class I loading and cell surface presentation to cytotoxic T cells. Here we delineate the mechanistic basis for antigen translocation. Notably, TAP works as a molecular diode, translocating peptide substrates against the gradient in a strict unidirectional way. We reveal the importance of the D-loop at the dimer interface of the two nucleotide-binding domains (NBDs) in coupling substrate translocation with ATP hydrolysis and defining transport vectoriality. Substitution of the conserved aspartate, which coordinates the ATP-binding site, decreases NBD dimerization affinity and turns the unidirectional primary active pump into a passive bidirectional nucleotide-gated facilitator. Thus, ATP hydrolysis is not required for translocation per se, but is essential for both active and unidirectional transport. Our data provide detailed mechanistic insight into how heterodimeric ABC exporters operate.
Mechanistic determinants of the directionality and energetics of active export by a heterodimeric ABC transporter.,Grossmann N, Vakkasoglu AS, Hulpke S, Abele R, Gaudet R, Tampe R Nat Commun. 2014 Nov 7;5:5419. doi: 10.1038/ncomms6419. PMID:25377891[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grossmann N, Vakkasoglu AS, Hulpke S, Abele R, Gaudet R, Tampe R. Mechanistic determinants of the directionality and energetics of active export by a heterodimeric ABC transporter. Nat Commun. 2014 Nov 7;5:5419. doi: 10.1038/ncomms6419. PMID:25377891 doi:http://dx.doi.org/10.1038/ncomms6419
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