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| - | | + | #REDIRECT [[7sp5]] This PDB entry is obsolete and replaced by 7sp5 |
| - | ==Crystal structure of a eukaryotic phosphate transporter==
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| - | <StructureSection load='4j05' size='340' side='right'caption='[[4j05]], [[Resolution|resolution]] 2.90Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[4j05]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aftol-id_612 Aftol-id 612]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J05 FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j05 OCA], [http://pdbe.org/4j05 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j05 RCSB], [http://www.ebi.ac.uk/pdbsum/4j05 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j05 ProSAT]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Phosphate is crucial for structural and metabolic needs, including nucleotide and lipid synthesis, signalling and chemical energy storage. Proton-coupled transporters of the major facilitator superfamily (MFS) are essential for phosphate uptake in plants and fungi, and also have a function in sensing external phosphate levels as transceptors. Here we report the 2.9 A structure of a fungal (Piriformospora indica) high-affinity phosphate transporter, PiPT, in an inward-facing occluded state, with bound phosphate visible in the membrane-buried binding site. The structure indicates both proton and phosphate exit pathways and suggests a modified asymmetrical 'rocker-switch' mechanism of phosphate transport. PiPT is related to several human transporter families, most notably the organic cation and anion transporters of the solute carrier family (SLC22), which are implicated in cancer-drug resistance. We modelled representative cation and anion SLC22 transporters based on the PiPT structure to surmise the structural basis for substrate binding and charge selectivity in this important family. The PiPT structure demonstrates and expands on principles of substrate transport by the MFS transporters and illuminates principles of phosphate uptake in particular.
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| - | Crystal structure of a eukaryotic phosphate transporter.,Pedersen BP, Kumar H, Waight AB, Risenmay AJ, Roe-Zurz Z, Chau BH, Schlessinger A, Bonomi M, Harries W, Sali A, Johri AK, Stroud RM Nature. 2013 Mar 31. doi: 10.1038/nature12042. PMID:23542591<ref>PMID:23542591</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4j05" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Aftol-id 612]]
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| - | [[Category: Large Structures]]
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| - | [[Category: Bonomi, M]]
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| - | [[Category: Chau, B H]]
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| - | [[Category: Harries, W]]
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| - | [[Category: Johri, A K]]
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| - | [[Category: Kumar, H]]
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| - | [[Category: Pedersen, B P]]
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| - | [[Category: Risenmay, A J]]
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| - | [[Category: Roe-Zurz, Z]]
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| - | [[Category: Sali, A]]
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| - | [[Category: Schlessinger, A]]
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| - | [[Category: Stroud, R M]]
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| - | [[Category: Waight, A B]]
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| - | [[Category: Major facilitator]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Phosphate binding]]
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| - | [[Category: Phosphate import]]
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| - | [[Category: Transport protein]]
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