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| | <StructureSection load='4led' size='340' side='right'caption='[[4led]], [[Resolution|resolution]] 2.37Å' scene=''> | | <StructureSection load='4led' size='340' side='right'caption='[[4led]], [[Resolution|resolution]] 2.37Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4led]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_str._c_1433 Pseudomonas aeruginosa str. c 1433]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LED FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4led]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_str._C_1433 Pseudomonas aeruginosa str. C 1433]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LED FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XXR:6-DEOXY-ALPHA-D-MANNOPYRANOSE'>XXR</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XXR:6-DEOXY-ALPHA-D-MANNOPYRANOSE'>XXR</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4le7|4le7]], [[4lea|4lea]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4led FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4led OCA], [https://pdbe.org/4led PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4led RCSB], [https://www.ebi.ac.uk/pdbsum/4led PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4led ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4led FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4led OCA], [http://pdbe.org/4led PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4led RCSB], [http://www.ebi.ac.uk/pdbsum/4led PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4led ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/T2LG16_PSEAI T2LG16_PSEAI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseudomonas aeruginosa str. c 1433]] | + | [[Category: Pseudomonas aeruginosa str. C 1433]] |
| - | [[Category: Cogdell, C J]] | + | [[Category: Cogdell CJ]] |
| - | [[Category: Grinter, R]] | + | [[Category: Grinter R]] |
| - | [[Category: Mccaughey, L]] | + | [[Category: Mccaughey L]] |
| - | [[Category: Roszak, A W]] | + | [[Category: Roszak AW]] |
| - | [[Category: Walker, D]] | + | [[Category: Walker D]] |
| - | [[Category: Antimicrobial protein]]
| + | |
| - | [[Category: Bacteriocin]]
| + | |
| - | [[Category: Beta prism]]
| + | |
| - | [[Category: Extracellular]]
| + | |
| - | [[Category: Galanthus nivalis agglutinin]]
| + | |
| - | [[Category: Monocot mannose binding protein]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
T2LG16_PSEAI
Publication Abstract from PubMed
Lectin-like bacteriocins consist of tandem monocot mannose-binding domains and display a genus-specific killing activity. Here we show that pyocin L1, a novel member of this family from Pseudomonas aeruginosa, targets susceptible strains of this species through recognition of the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide that is predominantly a homopolymer of d-rhamnose. Structural and biophysical analyses show that recognition of CPA occurs through the C-terminal carbohydrate-binding domain of pyocin L1 and that this interaction is a prerequisite for bactericidal activity. Further to this, we show that the previously described lectin-like bacteriocin putidacin L1 shows a similar carbohydrate-binding specificity, indicating that oligosaccharides containing d-rhamnose and not d-mannose, as was previously thought, are the physiologically relevant ligands for this group of bacteriocins. The widespread inclusion of d-rhamnose in the lipopolysaccharide of members of the genus Pseudomonas explains the unusual genus-specific activity of the lectin-like bacteriocins.
Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor.,McCaughey LC, Grinter R, Josts I, Roszak AW, Waloen KI, Cogdell RJ, Milner J, Evans T, Kelly S, Tucker NP, Byron O, Smith B, Walker D PLoS Pathog. 2014 Feb 6;10(2):e1003898. doi: 10.1371/journal.ppat.1003898., eCollection 2014 Feb. PMID:24516380[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ McCaughey LC, Grinter R, Josts I, Roszak AW, Waloen KI, Cogdell RJ, Milner J, Evans T, Kelly S, Tucker NP, Byron O, Smith B, Walker D. Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor. PLoS Pathog. 2014 Feb 6;10(2):e1003898. doi: 10.1371/journal.ppat.1003898., eCollection 2014 Feb. PMID:24516380 doi:http://dx.doi.org/10.1371/journal.ppat.1003898
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