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| | <StructureSection load='2v5v' size='340' side='right'caption='[[2v5v]], [[Resolution|resolution]] 1.88Å' scene=''> | | <StructureSection load='2v5v' size='340' side='right'caption='[[2v5v]], [[Resolution|resolution]] 1.88Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2v5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_pcc7119 Anabaena pcc7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V5V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2v5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena_pcc7119 Anabaena pcc7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V5V FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dx9|1dx9]], [[1ftg|1ftg]], [[1obo|1obo]], [[1obv|1obv]], [[1qhe|1qhe]], [[2v5u|2v5u]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dx9|1dx9]], [[1ftg|1ftg]], [[1obo|1obo]], [[1obv|1obv]], [[1qhe|1qhe]], [[2v5u|2v5u]]</div></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5v OCA], [http://pdbe.org/2v5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v5v RCSB], [http://www.ebi.ac.uk/pdbsum/2v5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5v ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5v OCA], [https://pdbe.org/2v5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v5v RCSB], [https://www.ebi.ac.uk/pdbsum/2v5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5v ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FLAV_NOSSO FLAV_NOSSO]] Low-potential electron donor to a number of redox enzymes. | + | [[https://www.uniprot.org/uniprot/FLAV_NOSSO FLAV_NOSSO]] Low-potential electron donor to a number of redox enzymes. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Flavodoxin|Flavodoxin]] | + | *[[Flavodoxin 3D structures|Flavodoxin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Structural highlights
Function
[FLAV_NOSSO] Low-potential electron donor to a number of redox enzymes.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Contribution of three regions (phosphate-binding, 50's and 90's loops) of Anabaena apoflavodoxin to FMN binding and reduction potential was studied. Thr12 and Glu16 did not influence FMN redox properties, but Thr12 played a role in FMN binding. Replacement of Trp57 with Glu, Lys or Arg moderately shifted E(ox/sq) and E(sq/hq) and altered the energetic of the FMN redox states binding profile. Our data indicate that the side chain of position 57 does not modulate E(ox/sq) by aromatic stacking or solvent exclusion, but rather by influencing the relative strength of the H-bond between the N(5) of the flavin and the Asn58-Ile59 bond. A correlation was observed between the isoalloxazine increase in solvent accessibility and less negative E(sq/hq). Moreover, E(sq/hq) became less negative as positively charged residues were added near to the isoalloxazine. Ile59 and Ile92 were simultaneously mutated to Ala or Glu. These mutations impaired FMN binding, while shifting E(sq/hq) to less negative values and E(ox/sq) to more negative. These effects are discussed on the bases of the X-ray structures of some of the Fld mutants, suggesting that in Anabaena Fld the structural control of both electron transfer steps is much more subtle than in other Flds.
Tuning of the FMN binding and oxido-reduction properties by neighboring side chains in Anabaena flavodoxin.,Frago S, Goni G, Herguedas B, Peregrina JR, Serrano A, Perez-Dorado I, Molina R, Gomez-Moreno C, Hermoso JA, Martinez-Julvez M, Mayhew SG, Medina M Arch Biochem Biophys. 2007 Nov 15;467(2):206-17. Epub 2007 Aug 29. PMID:17904516[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Frago S, Goni G, Herguedas B, Peregrina JR, Serrano A, Perez-Dorado I, Molina R, Gomez-Moreno C, Hermoso JA, Martinez-Julvez M, Mayhew SG, Medina M. Tuning of the FMN binding and oxido-reduction properties by neighboring side chains in Anabaena flavodoxin. Arch Biochem Biophys. 2007 Nov 15;467(2):206-17. Epub 2007 Aug 29. PMID:17904516 doi:10.1016/j.abb.2007.08.024
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