|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5lmj' size='340' side='right'caption='[[5lmj]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='5lmj' size='340' side='right'caption='[[5lmj]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lmj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LMJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LMJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lmj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LMJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fwo|5fwo]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lmj OCA], [http://pdbe.org/5lmj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lmj RCSB], [http://www.ebi.ac.uk/pdbsum/5lmj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lmj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lmj OCA], [https://pdbe.org/5lmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lmj RCSB], [https://www.ebi.ac.uk/pdbsum/5lmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lmj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| Line 21: |
Line 21: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Camelus glama]] | + | [[Category: Lama glama]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cambillau, C]] | + | [[Category: Cambillau C]] |
| - | [[Category: Leone, P]] | + | [[Category: Leone P]] |
| - | [[Category: Roussel, A]] | + | [[Category: Roussel A]] |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Nanobody]]
| + | |
| - | [[Category: Porphyromonas gingivali]]
| + | |
| Structural highlights
Publication Abstract from PubMed
PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM.
Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.,Duhoo Y, Roche J, Trinh TTN, Desmyter A, Gaubert A, Kellenberger C, Cambillau C, Roussel A, Leone P Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. doi:, 10.1107/S2053230X17005969. Epub 2017 Apr 26. PMID:28471361[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Duhoo Y, Roche J, Trinh TTN, Desmyter A, Gaubert A, Kellenberger C, Cambillau C, Roussel A, Leone P. Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis. Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. doi:, 10.1107/S2053230X17005969. Epub 2017 Apr 26. PMID:28471361 doi:http://dx.doi.org/10.1107/S2053230X17005969
|
