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| | <StructureSection load='4o5v' size='340' side='right'caption='[[4o5v]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='4o5v' size='340' side='right'caption='[[4o5v]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4o5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Theac Theac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O5V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4o5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum_DSM_1728 Thermoplasma acidophilum DSM 1728]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O5V FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o5v OCA], [https://pdbe.org/4o5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o5v RCSB], [https://www.ebi.ac.uk/pdbsum/4o5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o5v ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o6j|4o6j]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ta0872 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273075 THEAC])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o5v OCA], [http://pdbe.org/4o5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o5v RCSB], [http://www.ebi.ac.uk/pdbsum/4o5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o5v ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HJU1_THEAC Q9HJU1_THEAC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Theac]] | + | [[Category: Thermoplasma acidophilum DSM 1728]] |
| - | [[Category: Lee, J Y]] | + | [[Category: Lee JY]] |
| - | [[Category: Yeo, H K]] | + | [[Category: Yeo HK]] |
| - | [[Category: Ider]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| Structural highlights
Function
Q9HJU1_THEAC
Publication Abstract from PubMed
The iron-dependent regulator (IdeR) is a metal ion-activated transcriptional repressor that regulates the expression of genes encoding proteins involved in iron uptake to maintain metal-ion homeostasis. IdeR is a functional homologue of the diphtheria toxin repressor (DtxR), and both belong to the DtxR/MntR family of metalloregulators. The structure of Fe(2+)-bound IdeR (TA0872) from Themoplasma acidophilum was determined at 2.1 A resolution by X-ray crystallography using single-wavelength anomalous diffraction. The presence of Fe(2+), which is the true biological activator of IdeR, in the metal-binding site was ascertained by the use of anomalous difference electron-density maps using diffraction data collected at the Fe absorption edge. Each DtxR/IdeR subunit contains two metal ion-binding sites separated by 9 A, labelled the primary and ancillary sites, whereas the crystal structures of IdeR from T. acidophilum show a binuclear iron cluster separated by 3.2 A, which is novel to T. acidophilum IdeR. The metal-binding site analogous to the primary site in DtxR was unoccupied, and the ancillary site was occupied by binuclear clustered ions. This difference suggests that T. acidophilum IdeR and its closely related homologues are regulated by a mechanism distinct from that of either DtxR or MntR. T. acidophilum IdeR was also shown to have a metal-dependent DNA-binding property by electrophoretic mobility shift assay.
Structural analysis and insight into metal-ion activation of the iron-dependent regulator from Thermoplasma acidophilum.,Yeo HK, Park YW, Lee JY Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1281-8. doi:, 10.1107/S1399004714004118. Epub 2014 Apr 29. PMID:24816097[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yeo HK, Park YW, Lee JY. Structural analysis and insight into metal-ion activation of the iron-dependent regulator from Thermoplasma acidophilum. Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1281-8. doi:, 10.1107/S1399004714004118. Epub 2014 Apr 29. PMID:24816097 doi:http://dx.doi.org/10.1107/S1399004714004118
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