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| | <StructureSection load='4obv' size='340' side='right'caption='[[4obv]], [[Resolution|resolution]] 2.84Å' scene=''> | | <StructureSection load='4obv' size='340' side='right'caption='[[4obv]], [[Resolution|resolution]] 2.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4obv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rumgv Rumgv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OBV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4obv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_gnavus_ATCC_29149 Ruminococcus gnavus ATCC 29149]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OBV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OBV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2SU:ALPHA-(FLUOROMETHYL)-D-TRYPTOPHAN'>2SU</scene>, <scene name='pdbligand=3SO:{5-HYDROXY-4-[(1E)-4-(1H-INDOL-3-YL)-3-OXOBUT-1-EN-1-YL]-6-METHYLPYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>3SO</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2SU:ALPHA-(FLUOROMETHYL)-D-TRYPTOPHAN'>2SU</scene>, <scene name='pdbligand=3SO:{5-HYDROXY-4-[(1E)-4-(1H-INDOL-3-YL)-3-OXOBUT-1-EN-1-YL]-6-METHYLPYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>3SO</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4obu|4obu]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4obv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4obv OCA], [https://pdbe.org/4obv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4obv RCSB], [https://www.ebi.ac.uk/pdbsum/4obv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4obv ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RUMGNA_01526 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=411470 RUMGV])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4obv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4obv OCA], [http://pdbe.org/4obv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4obv RCSB], [http://www.ebi.ac.uk/pdbsum/4obv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4obv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TRPDC_RUMGV TRPDC_RUMGV] Catalyzes the decarboxylation of tryptophan to tryptamine. Tryptamine is a neurotransmitter that induces the release of serotonin, which is suggested to modulate gastrointestinal motility. Therefore, the tryptophan decarboxylase from the gut bacteria Ruminococcus gnavus (strain ATCC 29149 / VPI C7-9) may influence host brain and behavior. Has weak activity with tyrosine and phenylalanine.<ref>PMID:25263219</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Rumgv]] | + | [[Category: Fraser JS]] |
| - | [[Category: Benschoten, A H.Van]] | + | [[Category: Van Benschoten AH]] |
| - | [[Category: Fraser, J S]]
| + | |
| - | [[Category: Decarboxylase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Type 1 plp-dependent]]
| + | |
| Structural highlights
Function
TRPDC_RUMGV Catalyzes the decarboxylation of tryptophan to tryptamine. Tryptamine is a neurotransmitter that induces the release of serotonin, which is suggested to modulate gastrointestinal motility. Therefore, the tryptophan decarboxylase from the gut bacteria Ruminococcus gnavus (strain ATCC 29149 / VPI C7-9) may influence host brain and behavior. Has weak activity with tyrosine and phenylalanine.[1]
Publication Abstract from PubMed
Several recent studies describe the influence of the gut microbiota on host brain and behavior. However, the mechanisms responsible for microbiota-nervous system interactions are largely unknown. Using a combination of genetics, biochemistry, and crystallography, we identify and characterize two phylogenetically distinct enzymes found in the human microbiome that decarboxylate tryptophan to form the beta-arylamine neurotransmitter tryptamine. Although this enzymatic activity is exceedingly rare among bacteria more broadly, analysis of the Human Microbiome Project data demonstrate that at least 10% of the human population harbors at least one bacterium encoding a tryptophan decarboxylase in their gut community. Our results uncover a previously unrecognized enzymatic activity that can give rise to host-modulatory compounds and suggests a potential direct mechanism by which gut microbiota can influence host physiology, including behavior.
Discovery and Characterization of Gut Microbiota Decarboxylases that Can Produce the Neurotransmitter Tryptamine.,Williams BB, Van Benschoten AH, Cimermancic P, Donia MS, Zimmermann M, Taketani M, Ishihara A, Kashyap PC, Fraser JS, Fischbach MA Cell Host Microbe. 2014 Oct 8;16(4):495-503. doi: 10.1016/j.chom.2014.09.001., Epub 2014 Sep 25. PMID:25263219[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Williams BB, Van Benschoten AH, Cimermancic P, Donia MS, Zimmermann M, Taketani M, Ishihara A, Kashyap PC, Fraser JS, Fischbach MA. Discovery and Characterization of Gut Microbiota Decarboxylases that Can Produce the Neurotransmitter Tryptamine. Cell Host Microbe. 2014 Oct 8;16(4):495-503. doi: 10.1016/j.chom.2014.09.001., Epub 2014 Sep 25. PMID:25263219 doi:http://dx.doi.org/10.1016/j.chom.2014.09.001
- ↑ Williams BB, Van Benschoten AH, Cimermancic P, Donia MS, Zimmermann M, Taketani M, Ishihara A, Kashyap PC, Fraser JS, Fischbach MA. Discovery and Characterization of Gut Microbiota Decarboxylases that Can Produce the Neurotransmitter Tryptamine. Cell Host Microbe. 2014 Oct 8;16(4):495-503. doi: 10.1016/j.chom.2014.09.001., Epub 2014 Sep 25. PMID:25263219 doi:http://dx.doi.org/10.1016/j.chom.2014.09.001
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