|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4nss' size='340' side='right'caption='[[4nss]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='4nss' size='340' side='right'caption='[[4nss]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4nss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NSS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4nss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NSS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MSMEG_5817, MSMEI_5660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nss OCA], [https://pdbe.org/4nss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nss RCSB], [https://www.ebi.ac.uk/pdbsum/4nss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nss ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nss OCA], [http://pdbe.org/4nss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nss RCSB], [http://www.ebi.ac.uk/pdbsum/4nss PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nss ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0R4F7_MYCS2 A0R4F7_MYCS2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 23: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mycs2]] | + | [[Category: Mycolicibacterium smegmatis MC2 155]] |
| - | [[Category: Beddoe, T]] | + | [[Category: Beddoe T]] |
| - | [[Category: Brammananath, R]] | + | [[Category: Brammananath R]] |
| - | [[Category: Chan, P Y]] | + | [[Category: Chan PY]] |
| - | [[Category: Coppel, R L]] | + | [[Category: Coppel RL]] |
| - | [[Category: Crellin, P K]] | + | [[Category: Crellin PK]] |
| - | [[Category: Littler, D]] | + | [[Category: Littler D]] |
| - | [[Category: Rossjohn, J]] | + | [[Category: Rossjohn J]] |
| - | [[Category: Shahine, A E]] | + | [[Category: Shahine AE]] |
| - | [[Category: Mycobacterial survival]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
A0R4F7_MYCS2
Publication Abstract from PubMed
The success of pathogenic mycobacterial species is owing in part to their ability to parasitize the generally inhospitable phagosomal environment of host macrophages, utilizing a variety of strategies to avoid their antimycobacterial capabilities and thereby enabling their survival. A recently identified gene target in Mycobacterium smegmatis, highly conserved within Mycobacterium spp. and denoted MSMEG_5817, has been found to be important for bacterial survival within host macrophages. To gain insight into its function, the crystal structure of MSMEG_5817 has been solved to 2.40 A resolution. The structure reveals a high level of structural homology to the sterol carrier protein (SCP) family, suggesting a potential role of MSMEG_5817 in the binding and transportation of biologically relevant lipids required for bacterial survival. The lipid-binding capacity of MSMEG_5817 was confirmed by ELISA, revealing binding to a number of phospholipids with varying binding specificities compared with Homo sapiens SCP. A potential lipid-binding site was probed by alanine-scanning mutagenesis, revealing structurally relevant residues and a binding mechanism potentially differing from that of the SCPs.
A structural and functional investigation of a novel protein from Mycobacterium smegmatis implicated in mycobacterial macrophage survivability.,Shahine A, Littler D, Brammananath R, Chan PY, Crellin PK, Coppel RL, Rossjohn J, Beddoe T Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2264-76. doi:, 10.1107/S139900471401092X. Epub 2014 Aug 23. PMID:25195741[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shahine A, Littler D, Brammananath R, Chan PY, Crellin PK, Coppel RL, Rossjohn J, Beddoe T. A structural and functional investigation of a novel protein from Mycobacterium smegmatis implicated in mycobacterial macrophage survivability. Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2264-76. doi:, 10.1107/S139900471401092X. Epub 2014 Aug 23. PMID:25195741 doi:http://dx.doi.org/10.1107/S139900471401092X
|
