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| | <StructureSection load='4ofj' size='340' side='right'caption='[[4ofj]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='4ofj' size='340' side='right'caption='[[4ofj]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ofj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staac Staac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OFJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ofj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OFJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SACOL0217 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93062 STAAC])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ofj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ofj OCA], [https://pdbe.org/4ofj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ofj RCSB], [https://www.ebi.ac.uk/pdbsum/4ofj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ofj ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ofj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ofj OCA], [http://pdbe.org/4ofj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ofj RCSB], [http://www.ebi.ac.uk/pdbsum/4ofj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ofj ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/NIKA_STAA8 NIKA_STAA8] Part of the ABC transporter complex NikABCDE (Opp2) involved in nickel import. Binds nickel and transfers it to the membrane-bound permease. Required for full urease activity and plays a significant role in the virulence of S.aureus during urinary tract infection (UTI) (PubMed:20662775). May bind nickel via a nickel-chelator (PubMed:25611161).<ref>PMID:20662775</ref> <ref>PMID:25611161</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Staac]] | + | [[Category: Staphylococcus aureus subsp. aureus COL]] |
| - | [[Category: Cavazza, C]] | + | [[Category: Cavazza C]] |
| - | [[Category: Lebrette, H]] | + | [[Category: Lebrette H]] |
| - | [[Category: Abc-type importer]]
| + | |
| - | [[Category: Extracytoplasmic]]
| + | |
| - | [[Category: Extracytoplasmic nickel-binding protein]]
| + | |
| - | [[Category: Metal transport]]
| + | |
| - | [[Category: Nickel import]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
NIKA_STAA8 Part of the ABC transporter complex NikABCDE (Opp2) involved in nickel import. Binds nickel and transfers it to the membrane-bound permease. Required for full urease activity and plays a significant role in the virulence of S.aureus during urinary tract infection (UTI) (PubMed:20662775). May bind nickel via a nickel-chelator (PubMed:25611161).[1] [2]
Publication Abstract from PubMed
Staphylococcus aureus possesses two canonical ABC-importers dedicated to nickel acquisition: the NikABCDE and the CntABCDF systems, active under different growth conditions. This study reports on the extracytoplasmic nickel-binding components SaNikA and SaCntA. We showed by protein crystallography that SaNikA is able to bind either a Ni-(l-His)2 complex or a Ni-(l-His) (2-methyl-thiazolidine dicarboxylate) complex, depending on their availability in culture supernatants. Native mass spectrometry experiments on SaCntA revealed that it binds the Ni(ii) ion via a different histidine-dependent chelator but it cannot bind Ni-(l-His)2. In vitro experiments are consistent with in vivo nickel content measurements that showed that l-histidine has a high positive impact on nickel import via the Cnt system. These results suggest that although both systems may require free histidine, they use different strategies to import nickel.
Novel insights into nickel import in Staphylococcus aureus: the positive role of free histidine and structural characterization of a new thiazolidine-type nickel chelator.,Lebrette H, Borezee-Durant E, Martin L, Richaud P, Boeri Erba E, Cavazza C Metallomics. 2015 Jan 22. PMID:25611161[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hiron A, Posteraro B, Carriere M, Remy L, Delporte C, La Sorda M, Sanguinetti M, Juillard V, Borezee-Durant E. A nickel ABC-transporter of Staphylococcus aureus is involved in urinary tract infection. Mol Microbiol. 2010 Sep;77(5):1246-60. doi: 10.1111/j.1365-2958.2010.07287.x. PMID:20662775 doi:http://dx.doi.org/10.1111/j.1365-2958.2010.07287.x
- ↑ Lebrette H, Borezee-Durant E, Martin L, Richaud P, Boeri Erba E, Cavazza C. Novel insights into nickel import in Staphylococcus aureus: the positive role of free histidine and structural characterization of a new thiazolidine-type nickel chelator. Metallomics. 2015 Jan 22. PMID:25611161 doi:http://dx.doi.org/10.1039/c4mt00295d
- ↑ Lebrette H, Borezee-Durant E, Martin L, Richaud P, Boeri Erba E, Cavazza C. Novel insights into nickel import in Staphylococcus aureus: the positive role of free histidine and structural characterization of a new thiazolidine-type nickel chelator. Metallomics. 2015 Jan 22. PMID:25611161 doi:http://dx.doi.org/10.1039/c4mt00295d
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