6jq5
From Proteopedia
(Difference between revisions)
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- | '''Unreleased structure''' | ||
- | The | + | ==The structure of Hatchet Ribozyme== |
+ | <StructureSection load='6jq5' size='340' side='right'caption='[[6jq5]], [[Resolution|resolution]] 2.06Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[6jq5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JQ5 FirstGlance]. <br> | ||
+ | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jq5 OCA], [http://pdbe.org/6jq5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jq5 RCSB], [http://www.ebi.ac.uk/pdbsum/6jq5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jq5 ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Small self-cleaving ribozymes catalyze site-specific cleavage of their own phosphodiester backbone with implications for viral genome replication, pre-mRNA processing, and alternative splicing. We report on the 2.1-A crystal structure of the hatchet ribozyme product, which adopts a compact pseudosymmetric dimeric scaffold, with each monomer stabilized by long-range interactions involving highly conserved nucleotides brought into close proximity of the scissile phosphate. Strikingly, the catalytic pocket contains a cavity capable of accommodating both the modeled scissile phosphate and its flanking 5' nucleoside. The resulting modeled precatalytic conformation incorporates a splayed-apart alignment at the scissile phosphate, thereby providing structure-based insights into the in-line cleavage mechanism. We identify a guanine lining the catalytic pocket positioned to contribute to cleavage chemistry. The functional relevance of structure-based insights into hatchet ribozyme catalysis is strongly supported by cleavage assays monitoring the impact of selected nucleobase and atom-specific mutations on ribozyme activity. | ||
- | + | Hatchet ribozyme structure and implications for cleavage mechanism.,Zheng L, Falschlunger C, Huang K, Mairhofer E, Yuan S, Wang J, Patel DJ, Micura R, Ren A Proc Natl Acad Sci U S A. 2019 May 28;116(22):10783-10791. doi:, 10.1073/pnas.1902413116. Epub 2019 May 14. PMID:31088965<ref>PMID:31088965</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
+ | <div class="pdbe-citations 6jq5" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Ren, A]] | ||
+ | [[Category: Zheng, L]] | ||
+ | [[Category: Catalysis]] | ||
+ | [[Category: Cleavage]] | ||
+ | [[Category: Hatchet]] | ||
+ | [[Category: Non-coding rna]] | ||
+ | [[Category: Ribozyme]] | ||
+ | [[Category: Rna]] |
Revision as of 05:37, 12 June 2019
The structure of Hatchet Ribozyme
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Categories: Large Structures | Ren, A | Zheng, L | Catalysis | Cleavage | Hatchet | Non-coding rna | Ribozyme | Rna