6nc9
From Proteopedia
(Difference between revisions)
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<StructureSection load='6nc9' size='340' side='right'caption='[[6nc9]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='6nc9' size='340' side='right'caption='[[6nc9]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6nc9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NC9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Theab Theab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NC9 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">murJ, mviN, THA_1814 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=484019 THEAB])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nc9 OCA], [http://pdbe.org/6nc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nc9 RCSB], [http://www.ebi.ac.uk/pdbsum/6nc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nc9 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nc9 OCA], [http://pdbe.org/6nc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nc9 RCSB], [http://www.ebi.ac.uk/pdbsum/6nc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nc9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/MURJ_THEAB MURJ_THEAB]] Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.[HAMAP-Rule:MF_02078]<ref>PMID:28024149</ref> | [[http://www.uniprot.org/uniprot/MURJ_THEAB MURJ_THEAB]] Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.[HAMAP-Rule:MF_02078]<ref>PMID:28024149</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The biosynthesis of many polysaccharides, including bacterial peptidoglycan and eukaryotic N-linked glycans, requires transport of lipid-linked oligosaccharide (LLO) precursors across the membrane by specialized flippases. MurJ is the flippase for the lipid-linked peptidoglycan precursor Lipid II, a key player in bacterial cell wall synthesis, and a target of recently discovered antibacterials. However, the flipping mechanism of LLOs including Lipid II remains poorly understood due to a dearth of structural information. Here we report crystal structures of MurJ captured in inward-closed, inward-open, inward-occluded and outward-facing conformations. Together with mutagenesis studies, we elucidate the conformational transitions in MurJ that mediate lipid flipping, identify the key ion for function, and provide a framework for the development of inhibitors. | ||
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| + | Visualizing conformation transitions of the Lipid II flippase MurJ.,Kuk ACY, Hao A, Guan Z, Lee SY Nat Commun. 2019 Apr 15;10(1):1736. doi: 10.1038/s41467-019-09658-0. PMID:30988294<ref>PMID:30988294</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6nc9" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Theab]] | ||
[[Category: Kuk, A C.Y]] | [[Category: Kuk, A C.Y]] | ||
[[Category: Lee, S Y]] | [[Category: Lee, S Y]] | ||
Revision as of 09:20, 1 May 2019
Lipid II flippase MurJ, outward-facing conformation
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