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6aul

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Artificial Metalloproteins Containing a Co4O4 Active Site - 2xm-S112Y-b

Structural highlights

6aul is a 1 chain structure with sequence from Streptomyces avidinii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.36Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAV_STRAV The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Publication Abstract from PubMed

Artificial metalloproteins (ArMs) containing Co4O4 cubane active sites were constructed via biotin-streptavidin technology. Stabilized by hydrogen bonds (H-bonds), terminal and cofacial Co(III)-OH2 moieties are observed crystallographically in a series of immobilized cubane sites. Solution electrochemistry provided correlations of oxidation potential and pH. For variants containing Ser and Phe adjacent to the metallocofactor, 1e(-)/1H(+) chemistry predominates until pH 8, above which the oxidation becomes pH-independent. Installation of Tyr proximal to the Co4O4 active site provided a single H-bond to one of a set of cofacial Co(III)-OH2 groups. With this variant, multi-e(-)/multi-H(+) chemistry is observed, along with a change in mechanism at pH 9.5 that is consistent with Tyr deprotonation. With structural similarities to both the oxygen-evolving complex of photosystem II (H-bonded Tyr) and to thin film water oxidation catalysts (Co4O4 core), these findings bridge synthetic and biological systems for water oxidation, highlighting the importance of secondary sphere interactions in mediating multi-e(-)/multi-H(+) reactivity.

Artificial Metalloproteins Containing Co4O4 Cubane Active Sites.,Olshansky L, Huerta-Lavorie R, Nguyen AI, Vallapurackal J, Furst A, Tilley TD, Borovik AS J Am Chem Soc. 2018 Feb 13. doi: 10.1021/jacs.7b13052. PMID:29401385^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Olshansky L, Huerta-Lavorie R, Nguyen AI, Vallapurackal J, Furst A, Tilley TD, Borovik AS. Artificial Metalloproteins Containing Co4O4 Cubane Active Sites. J Am Chem Soc. 2018 Feb 13. doi: 10.1021/jacs.7b13052. PMID:29401385 doi:http://dx.doi.org/10.1021/jacs.7b13052

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6aul"

@@ Line 3: / Line 3: @@
 <StructureSection load='6aul' size='340' side='right'caption='[[6aul]], [[Resolution|resolution]] 1.36&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6aul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AUL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6aul]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AUL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.36&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aul OCA], [http://pdbe.org/6aul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aul RCSB], [http://www.ebi.ac.uk/pdbsum/6aul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aul ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6aul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aul OCA], [https://pdbe.org/6aul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6aul RCSB], [https://www.ebi.ac.uk/pdbsum/6aul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6aul ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
+[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: As 4 1583]]
 [[Category: Large Structures]]
-[[Category: Borovik, A S]]
+[[Category: Streptomyces avidinii]]
-[[Category: Huerta-Lavorie, R]]
+[[Category: Borovik AS]]
-[[Category: Nguyen, A I]]
+[[Category: Huerta-Lavorie R]]
-[[Category: Olshansky, L]]
+[[Category: Nguyen AI]]
-[[Category: Tilley, T D]]
+[[Category: Olshansky L]]
-[[Category: Vallapurakal, J]]
+[[Category: Tilley TD]]
-[[Category: Artificial metalloprotein]]
+[[Category: Vallapurakal J]]
-[[Category: Biomimetic]]
-[[Category: Biotin]]
-[[Category: Biotin-binding protein]]
-[[Category: Co4o4]]
-[[Category: Photosynthesis]]
-[[Category: Streptavidin]]
-[[Category: Water oxidation]]

6aul

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Current revision

Artificial Metalloproteins Containing a Co4O4 Active Site - 2xm-S112Y-b

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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