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| | <StructureSection load='4oom' size='340' side='right'caption='[[4oom]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='4oom' size='340' side='right'caption='[[4oom]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4oom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pa1r Pseudomonas aeruginosa pa1r]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OOM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4oom]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PA1R Pseudomonas aeruginosa PA1R]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OOM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2U3:(2Z)-2-(2-AMINO-1,3-THIAZOL-4-YL)-2-{[(1,5-DIHYDROXY-4-OXO-1,4-DIHYDROPYRIDIN-2-YL)METHOXY]IMINO}-N-{(2S)-1-HYDROXY-3-METHYL-3-[(SULFOOXY)AMINO]BUTAN-2-YL}ETHANAMIDE'>2U3</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2U3:(2Z)-2-(2-AMINO-1,3-THIAZOL-4-YL)-2-{[(1,5-DIHYDROXY-4-OXO-1,4-DIHYDROPYRIDIN-2-YL)METHOXY]IMINO}-N-{(2S)-1-HYDROXY-3-METHYL-3-[(SULFOOXY)AMINO]BUTAN-2-YL}ETHANAMIDE'>2U3</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ool|4ool]], [[4oon|4oon]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oom OCA], [https://pdbe.org/4oom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oom RCSB], [https://www.ebi.ac.uk/pdbsum/4oom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oom ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA1R_gp2318 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1279008 Pseudomonas aeruginosa PA1R])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidoglycan_glycosyltransferase Peptidoglycan glycosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.129 2.4.1.129] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oom OCA], [http://pdbe.org/4oom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oom RCSB], [http://www.ebi.ac.uk/pdbsum/4oom PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oom ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Cell division protein|Cell division protein]] | + | *[[Cell division protein 3D structures|Cell division protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Peptidoglycan glycosyltransferase]]
| + | [[Category: Pseudomonas aeruginosa PA1R]] |
| - | [[Category: Pseudomonas aeruginosa pa1r]] | + | [[Category: Caspers N]] |
| - | [[Category: Caspers, N]] | + | [[Category: Han S]] |
| - | [[Category: Han, S]] | + | [[Category: Knafels JD]] |
| - | [[Category: Knafels, J D]] | + | |
| - | [[Category: Pbp3]]
| + | |
| - | [[Category: Transferase-transferase inhibitor complex]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Multidrug-resistant Gram-negative pathogens are an emerging threat to human health, and addressing this challenge will require development of new antibacterial agents. This can be achieved through an improved molecular understanding of drug-target interactions combined with enhanced delivery of these agents to the site of action. Herein we describe the first application of siderophore receptor-mediated drug uptake of lactivicin analogues as a strategy that enables the development of novel antibacterial agents against clinically relevant Gram-negative bacteria. We report the first crystal structures of several sideromimic conjugated compounds bound to penicillin binding proteins PBP3 and PBP1a from Pseudomonas aeruginosa and characterize the reactivity of lactivicin and beta-lactam core structures. Results from drug sensitivity studies with beta-lactamase enzymes are presented, as well as a structure-based hypothesis to reduce susceptibility to this enzyme class. Finally, mechanistic studies demonstrating that sideromimic modification alters the drug uptake process are discussed.
Siderophore Receptor-Mediated Uptake of Lactivicin Analogues in Gram-Negative Bacteria.,Starr J, Brown MF, Aschenbrenner L, Caspers N, Che Y, Gerstenberger BS, Huband M, Knafels JD, Lemmon MM, Li C, McCurdy SP, McElroy E, Rauckhorst MR, Tomaras AP, Young JA, Zaniewski RP, Shanmugasundaram V, Han S J Med Chem. 2014 Apr 21. PMID:24694215[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Starr J, Brown MF, Aschenbrenner L, Caspers N, Che Y, Gerstenberger BS, Huband M, Knafels JD, Lemmon MM, Li C, McCurdy SP, McElroy E, Rauckhorst MR, Tomaras AP, Young JA, Zaniewski RP, Shanmugasundaram V, Han S. Siderophore Receptor-Mediated Uptake of Lactivicin Analogues in Gram-Negative Bacteria. J Med Chem. 2014 Apr 21. PMID:24694215 doi:http://dx.doi.org/10.1021/jm500219c
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