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| | <StructureSection load='4oht' size='340' side='right'caption='[[4oht]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='4oht' size='340' side='right'caption='[[4oht]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4oht]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_bei_nr-33708 Streptococcus pyogenes bei nr-33708]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OHT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4oht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_MGAS1882 Streptococcus pyogenes MGAS1882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OHT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ogd|4ogd]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oht OCA], [https://pdbe.org/4oht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oht RCSB], [https://www.ebi.ac.uk/pdbsum/4oht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oht ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gabD, MGAS1882_0809 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1010840 Streptococcus pyogenes BEI NR-33708])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oht OCA], [http://pdbe.org/4oht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oht RCSB], [http://www.ebi.ac.uk/pdbsum/4oht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oht ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0J9X1M8_STRPY A0A0J9X1M8_STRPY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Streptococcus pyogenes bei nr-33708]] | + | [[Category: Streptococcus pyogenes MGAS1882]] |
| - | [[Category: Chi, Y M]] | + | [[Category: Chi YM]] |
| - | [[Category: Jang, E H]] | + | [[Category: Jang EH]] |
| - | [[Category: Lee, K S]] | + | [[Category: Lee KS]] |
| - | [[Category: Park, S A]] | + | [[Category: Park SA]] |
| - | [[Category: Dehydrogenase]]
| + | |
| - | [[Category: Gabd]]
| + | |
| - | [[Category: Nadp binding]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| - | [[Category: Ssadh]]
| + | |
| Structural highlights
Function
A0A0J9X1M8_STRPY
Publication Abstract from PubMed
The gamma-Aminobutyric acid (GABA) that is found in prokaryotic and eukaryotic organisms has been used in various ways as a signaling molecule or a significant component generating metabolic energy under conditions of nutrient limitation or stress, through GABA catabolism. Succinic semialdehyde dehydrogenase (SSADH) catalyzes the oxidation of succinic semialdehyde to succinic acid in the final step of GABA catabolism. Here, we report the catalytic properties and two crystal structures of SSADH from Streptococcus pyogenes (SpSSADH) regarding its cofactor preference. Kinetic analysis showed that SpSSADH prefers NADP(+) over NAD(+) as a hydride acceptor. Moreover, the structures of SpSSADH were determined in an apo-form and in a binary complex with NADP(+) at 1.6 A and 2.1 A resolutions, respectively. Both structures of SpSSADH showed dimeric conformation, containing a single cysteine residue in the catalytic loop of each subunit. Further structural analysis and sequence comparison of SpSSADH with other SSADHs revealed that Ser158 and Tyr188 in SpSSADH participate in the stabilization of the 2'-phosphate group of adenine-side ribose in NADP(+). Our results provide structural insights into the cofactor preference of SpSSADH as the gram-positive bacterial SSADH.
Kinetic and Structural Characterization for Cofactor Preference of Succinic Semialdehyde Dehydrogenase from Streptococcus pyogenes.,Jang EH, Park SA, Chi YM, Lee KS Mol Cells. 2014 Oct 31;37(10):719-26. doi: 10.14348/molcells.2014.0162. Epub 2014, Sep 26. PMID:25256219[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Jang EH, Park SA, Chi YM, Lee KS. Kinetic and Structural Characterization for Cofactor Preference of Succinic Semialdehyde Dehydrogenase from Streptococcus pyogenes. Mol Cells. 2014 Oct 31;37(10):719-26. doi: 10.14348/molcells.2014.0162. Epub 2014, Sep 26. PMID:25256219 doi:http://dx.doi.org/10.14348/molcells.2014.0162
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