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| | <StructureSection load='4oi3' size='340' side='right'caption='[[4oi3]], [[Resolution|resolution]] 1.30Å' scene=''> | | <StructureSection load='4oi3' size='340' side='right'caption='[[4oi3]], [[Resolution|resolution]] 1.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4oi3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OI3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4oi3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OI3 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4oi6|4oi6]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oi3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oi3 OCA], [https://pdbe.org/4oi3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oi3 RCSB], [https://www.ebi.ac.uk/pdbsum/4oi3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oi3 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCO4226 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oi3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oi3 OCA], [http://pdbe.org/4oi3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oi3 RCSB], [http://www.ebi.ac.uk/pdbsum/4oi3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oi3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9FCE4_STRCO Q9FCE4_STRCO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strco]]
| + | [[Category: Chen Y]] |
| - | [[Category: Chen, Y]] | + | [[Category: Cheng W]] |
| - | [[Category: Cheng, W]] | + | [[Category: Jiang YL]] |
| - | [[Category: Jiang, Y L]] | + | [[Category: Lu M]] |
| - | [[Category: Lu, M]] | + | [[Category: Virolle MJ]] |
| - | [[Category: Virolle, M J]] | + | [[Category: Wang S]] |
| - | [[Category: Wang, S]] | + | [[Category: Zhang RG]] |
| - | [[Category: Zhang, R G]] | + | [[Category: Zhou CZ]] |
| - | [[Category: Zhou, C Z]] | + | |
| - | [[Category: A nickel responsive protein]]
| + | |
| - | [[Category: Ferredoxin-like fold]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Nickel binding]]
| + | |
| - | [[Category: Nickel responsive protein]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| Structural highlights
Function
Q9FCE4_STRCO
Publication Abstract from PubMed
The open reading frame SCO4226 of Streptomyces coelicolor A3(2) encodes an 82-residue hypothetical protein. Biochemical assays revealed that each SCO4226 dimer binds four nickel ions. To decipher the molecular function, we solved the crystal structures of SCO4226 in both apo- and nickel-bound (Ni-SCO4226) forms at 1.30 and 2.04 A resolution, respectively. Each subunit of SCO4226 dimer adopts a canonical ferredoxin-like fold with five beta-strands flanked by two alpha-helices. In the structure of Ni-SCO4226, four nickel ions are coordinated at the surface of the dimer. Further biochemical assays suggested that the binding of Ni2+ triggers the self-aggregation of SCO4226 in vitro. In addition, RT-qPCR assays demonstrated that the expression of SCO4226 gene in S. coelicolor is specifically up-regulated by the addition of Ni2+, but not other divalent ions such as Cu2+, Mn2+ or Co2+. All these results suggested that SCO4226 acts as a nickel binding protein, probably required for nickel sequestration and/or detoxification.
Streptomyces coelicolor SCO4226 Is a Nickel Binding Protein.,Lu M, Jiang YL, Wang S, Jin H, Zhang RG, Virolle MJ, Chen Y, Zhou CZ PLoS One. 2014 Oct 6;9(10):e109660. doi: 10.1371/journal.pone.0109660., eCollection 2014. PMID:25285530[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lu M, Jiang YL, Wang S, Jin H, Zhang RG, Virolle MJ, Chen Y, Zhou CZ. Streptomyces coelicolor SCO4226 Is a Nickel Binding Protein. PLoS One. 2014 Oct 6;9(10):e109660. doi: 10.1371/journal.pone.0109660., eCollection 2014. PMID:25285530 doi:http://dx.doi.org/10.1371/journal.pone.0109660
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