This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2jz0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2jz0.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2jz0.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2jz0| PDB=2jz0 | SCENE= }}
{{STRUCTURE_2jz0| PDB=2jz0 | SCENE= }}
-
'''DSX_short'''
+
===DSX_short===
-
==Overview==
+
<!--
-
The DSX (Doublesex) transcription factor regulates somatic sexual differentiation in Drosophila. Female and male isoforms (DSX(F) and DSX(M)) are formed due to sex-specific RNA splicing. DNA recognition, mediated by a shared N-terminal zinc module (the DM domain), is enhanced by a C-terminal dimerization element. Sex-specific extension of this element in DSX(F) and DSX(M) leads to assembly of distinct transcriptional preinitiation complexes. Here, we describe the structure of the extended C-terminal dimerization domain of DSX(F) as determined by multidimensional NMR spectroscopy. The core dimerization element is well ordered, giving rise to a dense network of interresidue nuclear Overhauser enhancements. The structure contains dimer-related UBA folds similar to those defined by x-ray crystallographic studies of a truncated domain. Whereas the proximal portion of the female tail extends helix 3 of the UBA fold, the distal tail is disordered. Ala substitutions in the proximal tail disrupt the sex-specific binding of IX (Intersex), an obligatory partner protein and putative transcriptional coactivator; IX-DSX(F) interaction is, by contrast, not disrupted by truncation of the distal tail. Mutagenesis of the UBA-like dimer of DSX(F) highlights the importance of steric and electrostatic complementarity across the interface. Two temperature-sensitive mutations at this interface have been characterized in yeast model systems. One weakens a network of solvated salt bridges, whereas the other perturbs the underlying nonpolar interface. These mutations confer graded gene-regulatory activity in yeast within a physiological temperature range and so may provide novel probes for genetic analysis of a sex-specific transcriptional program in Drosophila development.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18184648}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18184648 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18184648}}
==About this Structure==
==About this Structure==
-
2JZ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ0 OCA].
+
2JZ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ0 OCA].
==Reference==
==Reference==
Line 32: Line 36:
[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Ubiquitin]]
[[Category: Ubiquitin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 9 14:40:08 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:33:46 2008''

Revision as of 11:33, 27 July 2008

Image:2jz0.png

Template:STRUCTURE 2jz0

DSX_short

Template:ABSTRACT PUBMED 18184648

About this Structure

2JZ0 is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

Doublesex and the Regulation of Sexual Dimorphism in Drosophila melanogaster: STRUCTURE, FUNCTION, AND MUTAGENESIS OF A FEMALE-SPECIFIC DOMAIN., Yang Y, Zhang W, Bayrer JR, Weiss MA, J Biol Chem. 2008 Mar 14;283(11):7280-92. Epub 2008 Jan 9. PMID:18184648

Page seeded by OCA on Sun Jul 27 14:33:46 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jz0"
Personal tools