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2jz0

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{{STRUCTURE_2jz0| PDB=2jz0 | SCENE= }}
{{STRUCTURE_2jz0| PDB=2jz0 | SCENE= }}
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'''DSX_short'''
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===DSX_short===
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==Overview==
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The DSX (Doublesex) transcription factor regulates somatic sexual differentiation in Drosophila. Female and male isoforms (DSX(F) and DSX(M)) are formed due to sex-specific RNA splicing. DNA recognition, mediated by a shared N-terminal zinc module (the DM domain), is enhanced by a C-terminal dimerization element. Sex-specific extension of this element in DSX(F) and DSX(M) leads to assembly of distinct transcriptional preinitiation complexes. Here, we describe the structure of the extended C-terminal dimerization domain of DSX(F) as determined by multidimensional NMR spectroscopy. The core dimerization element is well ordered, giving rise to a dense network of interresidue nuclear Overhauser enhancements. The structure contains dimer-related UBA folds similar to those defined by x-ray crystallographic studies of a truncated domain. Whereas the proximal portion of the female tail extends helix 3 of the UBA fold, the distal tail is disordered. Ala substitutions in the proximal tail disrupt the sex-specific binding of IX (Intersex), an obligatory partner protein and putative transcriptional coactivator; IX-DSX(F) interaction is, by contrast, not disrupted by truncation of the distal tail. Mutagenesis of the UBA-like dimer of DSX(F) highlights the importance of steric and electrostatic complementarity across the interface. Two temperature-sensitive mutations at this interface have been characterized in yeast model systems. One weakens a network of solvated salt bridges, whereas the other perturbs the underlying nonpolar interface. These mutations confer graded gene-regulatory activity in yeast within a physiological temperature range and so may provide novel probes for genetic analysis of a sex-specific transcriptional program in Drosophila development.
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(as it appears on PubMed at http://www.pubmed.gov), where 18184648 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18184648}}
==About this Structure==
==About this Structure==
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2JZ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ0 OCA].
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2JZ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ0 OCA].
==Reference==
==Reference==
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[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Ubiquitin]]
[[Category: Ubiquitin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 9 14:40:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:33:46 2008''

Revision as of 11:33, 27 July 2008

Image:2jz0.png

Template:STRUCTURE 2jz0

DSX_short

Template:ABSTRACT PUBMED 18184648

About this Structure

2JZ0 is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

Doublesex and the Regulation of Sexual Dimorphism in Drosophila melanogaster: STRUCTURE, FUNCTION, AND MUTAGENESIS OF A FEMALE-SPECIFIC DOMAIN., Yang Y, Zhang W, Bayrer JR, Weiss MA, J Biol Chem. 2008 Mar 14;283(11):7280-92. Epub 2008 Jan 9. PMID:18184648

Page seeded by OCA on Sun Jul 27 14:33:46 2008

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