|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4p5j' size='340' side='right'caption='[[4p5j]], [[Resolution|resolution]] 1.99Å' scene=''> | | <StructureSection load='4p5j' size='340' side='right'caption='[[4p5j]], [[Resolution|resolution]] 1.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4p5j]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P5J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4p5j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Turnip_yellow_mosaic_virus Turnip yellow mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P5J FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p5j OCA], [https://pdbe.org/4p5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p5j RCSB], [https://www.ebi.ac.uk/pdbsum/4p5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p5j ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p5j OCA], [http://pdbe.org/4p5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p5j RCSB], [http://www.ebi.ac.uk/pdbsum/4p5j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p5j ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| Line 22: |
Line 21: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Colussi, T M]] | + | [[Category: Turnip yellow mosaic virus]] |
| - | [[Category: Costantino, D A]] | + | [[Category: Colussi TM]] |
| - | [[Category: Hammond, J A]] | + | [[Category: Costantino DA]] |
| - | [[Category: Kieft, J S]] | + | [[Category: Hammond JA]] |
| - | [[Category: Nix, J C]] | + | [[Category: Kieft JS]] |
| - | [[Category: Ruehle, G M]] | + | [[Category: Nix JC]] |
| - | [[Category: Rna]] | + | [[Category: Ruehle GM]] |
| - | [[Category: Trna-mimic viral rna pseudoknot multifunctional]]
| + | |
| Structural highlights
Publication Abstract from PubMed
RNA is arguably the most functionally diverse biological macromolecule. In some cases a single discrete RNA sequence performs multiple roles, and this can be conferred by a complex three-dimensional structure. Such multifunctionality can also be driven or enhanced by the ability of a given RNA to assume different conformational (and therefore functional) states. Despite its biological importance, a detailed structural understanding of the paradigm of RNA structure-driven multifunctionality is lacking. To address this gap it is useful to study examples from single-stranded positive-sense RNA viruses, a prototype being the tRNA-like structure (TLS) found at the 3' end of the turnip yellow mosaic virus (TYMV). This TLS not only acts like a tRNA to drive aminoacylation of the viral genomic (g)RNA, but also interacts with other structures in the 3' untranslated region of the gRNA, contains the promoter for negative-strand synthesis, and influences several infection-critical processes. TLS RNA can provide a glimpse into the structural basis of RNA multifunctionality and plasticity, but for decades its high-resolution structure has remained elusive. Here we present the crystal structure of the complete TYMV TLS to 2.0 A resolution. Globally, the RNA adopts a shape that mimics tRNA, but it uses a very different set of intramolecular interactions to achieve this shape. These interactions also allow the TLS to readily switch conformations. In addition, the TLS structure is 'two faced': one face closely mimics tRNA and drives aminoacylation, the other face diverges from tRNA and enables additional functionality. The TLS is thus structured to perform several functions and interact with diverse binding partners, and we demonstrate its ability to specifically bind to ribosomes.
The structural basis of transfer RNA mimicry and conformational plasticity by a viral RNA.,Colussi TM, Costantino DA, Hammond JA, Ruehle GM, Nix JC, Kieft JS Nature. 2014 Jun 8. doi: 10.1038/nature13378. PMID:24909993[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Colussi TM, Costantino DA, Hammond JA, Ruehle GM, Nix JC, Kieft JS. The structural basis of transfer RNA mimicry and conformational plasticity by a viral RNA. Nature. 2014 Jun 8. doi: 10.1038/nature13378. PMID:24909993 doi:http://dx.doi.org/10.1038/nature13378
|
