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4p2v

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Revision as of 12:38, 1 February 2023

Structure of the AI-2 processing enzyme LsrF in complex with the product of the LsrG reaction P-HPD

Structural highlights

4p2v is a 10 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LSRF_ECOLI Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle (By similarity).

Publication Abstract from PubMed

The quorum sensing signal autoinducer-2 (AI-2) regulates important bacterial behaviors, including biofilm formation and the production of virulence factors. Some bacteria, such as Escherichia coli, can quench the AI-2 signal produced by a variety of species present in the environment, and thus can influence AI-2-dependent bacterial behaviors. This process involves uptake of AI-2 via the Lsr transporter, followed by phosphorylation and consequent intracellular sequestration. Here we determine the metabolic fate of intracellular AI-2 by characterizing LsrF, the terminal protein in the Lsr AI-2 processing pathway. We identify the substrates of LsrF as 3-hydroxy-2,4-pentadione-5-phosphate (P-HPD, an isomer of AI-2-phosphate) and coenzyme A, determine the crystal structure of an LsrF catalytic mutant bound to P-HPD, and identify the reaction products. We show that LsrF catalyzes the transfer of an acetyl group from P-HPD to coenzyme A yielding dihydroxyacetone phosphate and acetyl-CoA, two key central metabolites. We further propose that LsrF, despite strong structural homology to aldolases, acts as a thiolase, an activity previously undescribed for this family of enzymes. With this work, we have fully characterized the biological pathway for AI-2 processing in E. coli, a pathway that can be used to quench AI-2 and control quorum-sensing-regulated bacterial behaviors.

LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.,Marques JC, Oh IK, Ly DC, Lamosa P, Ventura MR, Miller ST, Xavier KB Proc Natl Acad Sci U S A. 2014 Sep 15. pii: 201408691. PMID:25225400^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Marques JC, Oh IK, Ly DC, Lamosa P, Ventura MR, Miller ST, Xavier KB. LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2. Proc Natl Acad Sci U S A. 2014 Sep 15. pii: 201408691. PMID:25225400 doi:http://dx.doi.org/10.1073/pnas.1408691111

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4p2v"

Categories: Escherichia coli K-12 | Large Structures | Miller ST | Oh IK | Xavier KB

@@ Line 3: / Line 3: @@
 <StructureSection load='4p2v' size='340' side='right'caption='[[4p2v]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4p2v]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P2V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4p2v]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P2V FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=26T:(3R)-3-HYDROXY-2,4-DIOXOPENTYL+DIHYDROGEN+PHOSPHATE'>26T</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=26T:(3R)-3-HYDROXY-2,4-DIOXOPENTYL+DIHYDROGEN+PHOSPHATE'>26T</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lsrF, yneB, b1517, JW1510 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p2v OCA], [https://pdbe.org/4p2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p2v RCSB], [https://www.ebi.ac.uk/pdbsum/4p2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p2v ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p2v OCA], [http://pdbe.org/4p2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p2v RCSB], [http://www.ebi.ac.uk/pdbsum/4p2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p2v ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LSRF_ECOLI LSRF_ECOLI]] Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle (By similarity).
+[https://www.uniprot.org/uniprot/LSRF_ECOLI LSRF_ECOLI] Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 18: @@
 </div>
 <div class="pdbe-citations 4p2v" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Aldolase 3D structures|Aldolase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Miller, S T]]
+[[Category: Miller ST]]
-[[Category: Oh, I K]]
+[[Category: Oh IK]]
-[[Category: Xavier, K B]]
+[[Category: Xavier KB]]
-[[Category: Lyase]]
-[[Category: Thiolase]]

4p2v

From Proteopedia

Revision as of 12:38, 1 February 2023

Structure of the AI-2 processing enzyme LsrF in complex with the product of the LsrG reaction P-HPD

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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