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6jhy
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein== | |
| + | <StructureSection load='6jhy' size='340' side='right'caption='[[6jhy]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jhy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JHY FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jhy OCA], [http://pdbe.org/6jhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jhy RCSB], [http://www.ebi.ac.uk/pdbsum/6jhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jhy ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The DcCoV UAE-HKU23 coronavirus is a newly-found betacoronavirus (betaCoV) that can infect human cells. The viral spike protein plays pivotal roles in mediating receptor-recognition and membrane-fusion, and is therefore a key factor involved in viral pathogenesis and inter-species transmission. Here we reported the structural and functional characterization of the spike N-terminal domain (NTD) from DcCoV UAE-HKU23 (HKU23-NTD). Via mucin-binding assays, we showed that HKU23-NTD is able to bind sugars. We further solved the structure of HKU23-NTD, performed structure-guided mutagenesis and successfully located the potential sugar-binding pockets in the structure. Furthermore, via comparison of available betaCoV NTD structures, we demonstrated that betaCoV NTDs contain a conserved beta-sandwich core, but exhibit variant folds in the peripheral elements located in the top-ceiling region and on the lateral side. While showing different compositions and structures, these peripheral elements are topologically equivalent beta-sandwich-core insertions, highlighting a divergent evolution process for betaCoVs to form different lineages. | ||
| - | + | Crystal structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein.,Cheng Y, He B, Yang J, Ye F, Lin S, Yang F, Chen Z, Chen Z, Cao Y, Lu G Virology. 2019 Jun 27;535:74-82. doi: 10.1016/j.virol.2019.06.015. PMID:31279241<ref>PMID:31279241</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6jhy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cheng, Y]] | ||
| + | [[Category: Lu, G]] | ||
| + | [[Category: Ye, F]] | ||
| + | [[Category: Crystal structure]] | ||
| + | [[Category: Dccov uae-hku23]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 06:13, 24 July 2019
Crystal Structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein
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